Hi,

I have my gene of interest in pcDNA5/FRT/TO and would like to generate a stable cell line. Before generating the stable line, however, I would like to test expression by transient transfection.

 Can the pcDNA5FRT/TO vector be used for transient expression? If so, do I need to add doxycycline to induce expression (as would be done with a stable line ...) or is the repressor only active when integrated into the genome?

 In the handful of papers that I found, addition of doxycyclin during transient overexpression of a construct in pcDNA5/FRT/TO was not specifically mentioned.

 Thank you,

 Norbert

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