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Questions related from Norbert Volkmar
Is there an easy way to deliver lipids in vitro to crude membrane preparations? The membranes have been prepared from cell lysates by ultracentrifugation in non-denaturing, detergent-free buffer....
16 November 2022 8,221 0 View
I was wondering if anyone could relate their experience with isolating and analysing protein complexes in complex samples (whole-cell lysates) using mass spectrometry on sucrose gradient...
17 March 2022 9,958 5 View
I noticed that in many protocols for the isolation of detergent-resistant proteins/aggregates sucrose is added either directly to the lysis buffer or the the lysate is centrifuged through a...
30 August 2021 7,672 1 View
I am aiming to isolate microsomes from a cell line and then alter their lipid composition in vitro by artificially introducing lipids such as choesterol or phospholipids. The membrane proteins...
09 January 2021 5,908 1 View
Has anyone done gene knockout using CRISPR/Cas9 in SGBS preadipocytes? Are these cells amenable to CRISPRX manipulation?
20 March 2019 7,382 1 View
Hi, Can anyone recommend a specific mammalian expression vector that I could use to tag my protein of interest and that would allow me to purify any associated complexes for MS analysis? I am...
14 July 2014 5,500 10 View
Hi, I have my gene of interest in pcDNA5/FRT/TO and would like to generate a stable cell line. Before generating the stable line, however, I would like to test expression by transient...
07 July 2014 212 3 View
I am experiencing problems with one-dimensional Blue Native PAGE: It seems that proteins in the rage of 300 - 600 kDa are not properly transferred onto the PVDF membrane during Western Blotting of...
05 July 2014 9,491 5 View
