Norbert Volkmar

5 Questions 5 Answers 0 Followers

Questions related from Norbert Volkmar

In vitro lipid / cholesterol delivery to membrane preparations?

Is there an easy way to deliver lipids in vitro to crude membrane preparations? The membranes have been prepared from cell lysates by ultracentrifugation in non-denaturing, detergent-free buffer....

16 November 2022 8,212 0 View

Sucrose gradients and mass spectrometry to analyse protein complexes?

I was wondering if anyone could relate their experience with isolating and analysing protein complexes in complex samples (whole-cell lysates) using mass spectrometry on sucrose gradient...

17 March 2022 9,938 5 View

Mammalian expression vector for isolation of native protein complexes?

Hi, Can anyone recommend a specific mammalian expression vector that I could use to tag my protein of interest and that would allow me to purify any associated complexes for MS analysis? I am...

14 July 2014 5,486 10 View

Can the pcDNA5FRT/TO vector be used for transient overexpression of a gene of interest?

Hi, I have my gene of interest in pcDNA5/FRT/TO and would like to generate a stable cell line. Before generating the stable line, however, I would like to test expression by transient...

07 July 2014 199 3 View

Problem with Blue Native PAGE: Why is the transfer incomplete?

I am experiencing problems with one-dimensional Blue Native PAGE: It seems that proteins in the rage of 300 - 600 kDa are not properly transferred onto the PVDF membrane during Western Blotting of...

05 July 2014 9,479 5 View