Gene synthesis - pros and cons of codon optimization?

Dear all, I am synthesizing a nematode gene for reexpression in human cells and functional characterization (not for protein expression/purification). What are the pros and cons of codon...

04 November 2019 2,399 2 View

How to estimate regression to find which firm-specific characteristics explain cross-industry heterogeneity in the firm returns?

I would kindly like to ask for guidance regarding my question below. To illustrate my question: I have two industries: Industry 1 and Industry 2. Both industries contain a number of firms that...

20 May 2019 4,907 1 View

Is there a method to make a serum working on paraffin embedded tissue?

We have raised a rabbit antiserum against a protein coupled peptide. This peptide has been reported to be antigenic and some papers have been published with a commercial preparation raised in the...

22 January 2017 5,681 4 View

Can anyone recommend antibodies for histochemistry of rat macrophages and B-cells?

We try to detect macrophages and B-cells in rat bone-tissue. However, we get cross reaction when using mouse monoclonals. Has anybody experienced similar problems and developed a solution?

15 December 2016 2,972 4 View

Which exon should I target for CRISPR-Cas9 system?

Is there any rule/preference which exon should I target to knock out the GOI by CRISPR-Cas9 system?

13 November 2016 4,728 5 View

Who has experience with activity assays for FOLH1?

We have observed a massive transcriptional regulation of FOLH1 in cell cultures. This protein is also known as Prostate-Specific Membrane Antigen or Glutamate Carboxypeptidase II. However we were...

25 October 2016 4,658 2 View

Does anybody know a simplified methodology or equation to estimate the temperature of a solar collector/absorber?

Does anybody know a simplified methodology or equation to estimate the temperature of a solar collector/absorber? I want to calculate the energy performance and the energy yield. I have Data for a...

17 March 2016 4,911 5 View

Does my ligated plasmid ready for sequencing and is the purity OK?

I performed a ligation of 1.5 kb insert into a 4.2 kb vector and I got colonies on my kanamycin plate. I picked up 8 colonies and made a miniprep of them. 2 out of the 8 samples contained the...

15 February 2016 860 3 View

How do we solve our problem with mouse hippocampal cell cultures on 35 mm petri dishes?

Hi there,We have some problems with (E17-18) mouse hippocampal cell cultures. After sacrificing mice (E17-18), primary neurons were cultured (400,000 cells per on the 35 mm petri dishes). For...

08 February 2015 7,460 6 View

Mammalian expression vector for isolation of native protein complexes?

Hi, Can anyone recommend a specific mammalian expression vector that I could use to tag my protein of interest and that would allow me to purify any associated complexes for MS analysis? I am...

13 July 2014 5,410 10 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

What is the best solvent to remove PDMS from nano particles?

I want to remove PDMS oligomers from the surface of nanolayers. I have just read a very helpful paper about the compatibility of PDMS with different solvents( Solvent Compatibility of...

24 February 2021 7,845 5 View