How to indicate the degree of litter decomposed by microbes?

Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...

23 February 2021 1,362 5 View

How to improve dissolution of neosome?

I have made neosome from herbal drug. Neosomes are stable. But I face problems in dissolution. I used minimum ratio of 1:1:0.25 ( Drug:span 60: Cholesterol and GMs. Now how can I active better...

17 February 2021 3,584 1 View

Hi, could anyone suggest any mammalian expression plasmid that has a GFP as a selection marker?

I am looking for a plasmid that can express my gene of interest without GFP fusion. But I want its selection marker like neomycin replaced by GFP or any other fluorescent protein so that I can...

29 October 2020 2,247 3 View

Predict charge on a compound at different pH?

Dear fellow scientists, Do you know of a software or a resource base which can predict the charge on the analyte at different pH conditions? Thank you, Abhishek P.S. I am new to HPLC, even a...

19 October 2020 8,605 3 View

How to define Darcy's flow for pore pressure cohesive element in Abaqus?

Hi everyone, I am trying to define a Darcy's flow for pore pressure cohesive element in Abaqus. When I give permeability to the elements, the output of tangential flow rate (GFVR) is always shown...

11 October 2020 1,195 3 View

If one publishes in a journal with impact factor >1.4 and which is not included in NAAS list of journals, will the paper be equally valued by ICAR?

I understand the difference between NAAS score and Impact Factor but is it okay to publish in a non-NAAS, high IF journal? How will it be valued in ICAR interviews and related recruitments?

10 September 2020 7,575 3 View

What is the model equation between resistivity and temperature of a extrinsic semiconductor?

Can anyone Tell me what is the model relation between resistivity and the temperature for a extrinsic semiconductor. Kindly provide with summarized clarification & model equation between...

30 August 2020 6,182 11 View

What is timely Supply chain management issues to be researched as a result of the COVID 19 pandemic?

Dear Research Scholars, Would you please share your opinion on "What is timely Supply chain management issues to be researched as a result of the COVID 19 pandemic?" Thanks Shuvro Sen

22 July 2020 6,995 15 View

Can anyone help me to possibly identify the following fish?

A pair of damselfish were collected by an acquaintance in the Gulf of Mannar area, India. Can anyone help in the identification? P.S. Sorry for the out of focus pics.

01 July 2020 5,068 5 View

Does anyone have a case study of kick/influx and fluid losses during drilling, while MPD (managed pressure drilling) system is active?

Looking for a case study where severe influx and loss events were experienced even when a MPD system is installed and active. Interested in the sequence of events and mitigation steps. A real well...

12 June 2020 8,922 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

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28 February 2021 9,936 2 View