Priyankar Sen

7 Questions 23 Answers 0 Followers

Questions related from Priyankar Sen

What are the factors taken care of during purification of unstable proteins?

Theoretically, my protein is unstable. till DNA level everything is quite fine, but I think I am losing it during heating with bromophenol for SDS-PAGE. Any suggestion?

19 April 2016 522 4 View

Why is my Tris buffer freezing even 8-10 degree Celcius?

Why 100mM Tris HCl buffer pH 7 freezes even 8-10 degree celcius?

08 May 2015 196 6 View

Why inclusion bodies generally have only one protein?

It is told that mostly inclusion bodies possess only one protein. but why? why the kinetics of aggregation attracts only one protein?

23 January 2015 7,321 4 View

Is anyone familiar with polyproline tags for purification?

Hello friends, I have a protein (without his-tag) naturally having one pentaproline sequence in the solvent accessible position. Does anybody have experience with purification using the help of...

20 November 2014 9,262 1 View

How can I decrease temperature in 5 liter fermenter quickly?

Currently I am working with a locally made fully manual 5 liter fermenter (Scigenics, chennai). After sterilization, it use to take a lot of time to cool down to RT. Is there any way to decrease...

22 May 2014 8,876 5 View

Why is dissolved oxygen decreasing?

I am growing e.coli in fermenter with glucose in minimal media at pH 7.4 (50mM SPB). Technically, first D.O. should decrease (to nearly zero) due to cell growth, and then it should increase, due...

30 April 2013 1,485 8 View

Can start site be predicted?

The interest of my DNA sequence has 11 codons for methionine. Can I predict from which one protein synthesis will start?

20 February 2013 4,260 3 View