Ruhul Amin

6 Questions 35 Answers 0 Followers

Questions related from Ruhul Amin

Is there any good prognostic marker to predict late recurrence of breast cancer?

There are some markers to predict early recurrence in less than 5 years. But certain subtypes of breast cancer (ER+, luminal) has been shown to undergo relapse in 10-20 years. I was wondering...

07 March 2015 9,398 8 View

Which normalization method is better for microRNA microarray data analysis?

Recently we performed miRNA microarray and we found that many novel miRNA candidates are present in our data. Although many of them have high expression value, their functions are still undefined...

24 August 2014 8,072 5 View

What are the good markers for G2/M phase of cell cycle?

I was getting high G2/M population in PI staining. I was looking for a specific marker (FC/IF/WB) of G2/M transition. Cyclin B1 and CDK1 usually acts in this stage. Can anyone please suggest a marker?

21 April 2014 8,713 13 View

Is Epithelial-mesenchymal transition (EMT) a dedifferentiated state of the cell?

EMT generate cells with stem like properties. I was wondering whether it reverses the the differentiated cells into undifferentiated state? My cells undergo dedifferentiation and acquires cancer...

20 March 2014 6,703 3 View

Has anyone used RPMI medium for mammosphere formation assay?

Usually DMEM/F12 is recommended for Mammosphere formation assay. But I've grown some of my cell line in RPMI medium. I was wondering if I transfer them into DMEM/F12, how do they react? Is that OK...

27 March 2013 2,144 3 View

Is it possible for a single microRNA to act differently depending on cell types?

I mean within the same cancer but different stages ( primary, metastatic), can a single microRNA act as oncogenic or tumor suppressor?

03 January 2013 9,511 8 View