Has anyone performed novel object recognition and fear conditioning in the same animals?

When testing memory and learning, did anyone combine these tests in the same set of animals? What could be the dynamics in performing these test? What could be pros/cons for doing this kind of...

01 February 2018 483 0 View

Transport of isolated RNA?

After isolated of total RNA from blood lymphocytes what is the best way of transporting the RNA? After dissolving in DEPT-treated water what is the optimal way? Should the samples be on ice or what?

04 May 2016 6,826 4 View

Connection between expression of 5ht1a and p11 gene upon stress and AD?

04 May 2016 3,312 0 View

How to do behavioral tests?

What is the time schedule for performing the behavioral tests after ICV application of DEX in mice? How to perform FST: in two sessions or in one, when to do sucrose and what is the time table for...

03 April 2016 382 2 View

Any suggestion of a whole cell western blot protocol from rat testis?

Dear, Anyone has/tried some protocol for extraction of whole cell from rat testis for western blot? Which buffer you used, troubleshooting and similar things.

11 December 2015 786 5 View

How doI dissolve DEX-BSA for intracerebroventricular injections?

09 October 2015 4,774 0 View

Noldus EthoVision Xt multiple footshocks setup?

How to setup multiple foot shocks in Noldus EthoVision XT software? I need to adjust three footshocks in 1,58, 2,28 and 2,58 second from the beging of conditioning! Fs last for 2 seconds?

09 October 2015 8,044 0 View

Freezing behavior statistics?

07 August 2015 2,021 4 View

Does DNase treatment affect the concentration and purity of isolated RNA?

Namely, in our lab when we measured concentration of RNA sample after and without DNAse treatment, we got surprising results. RNA concentration of sample after DNAse treatment was 5 fold lower...

11 December 2014 5,813 7 View

No title

SYBR Green Real Time PCR

07 August 2014 7,368 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View