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Questions related from Milos Mitic
When testing memory and learning, did anyone combine these tests in the same set of animals? What could be the dynamics in performing these test? What could be pros/cons for doing this kind of...
02 February 2018 539 0 View
After isolated of total RNA from blood lymphocytes what is the best way of transporting the RNA? After dissolving in DEPT-treated water what is the optimal way? Should the samples be on ice or what?
05 May 2016 6,867 4 View
We know that p11 transports the 5-HT1B receptor from the cytoplasm to the membrane and increases density of this receptor on postsynaptic membrane and therefore upregulates serotonergic...
05 May 2016 3,359 0 View
What is the time schedule for performing the behavioral tests after ICV application of DEX in mice? How to perform FST: in two sessions or in one, when to do sucrose and what is the time table for...
04 April 2016 432 2 View
Dear, Anyone has/tried some protocol for extraction of whole cell from rat testis for western blot? Which buffer you used, troubleshooting and similar things.
12 December 2015 846 5 View
How to setup multiple foot shocks in Noldus EthoVision XT software? I need to adjust three footshocks in 1,58, 2,28 and 2,58 second from the beging of conditioning! Fs last for 2 seconds?
10 October 2015 8,105 0 View
Is it just saline or for example CSF or is it combination of DMSO and CSF for instance? Anyone had a problem regarding the infusion of DEX-BSA?
10 October 2015 4,827 0 View
When you have WT & KO mice and you do a fear extinction experiment in 7 days period, which statistics is appropriate to use to differences between genotype and inter-group differences between...
08 August 2015 2,088 4 View
Namely, in our lab when we measured concentration of RNA sample after and without DNAse treatment, we got surprising results. RNA concentration of sample after DNAse treatment was 5 fold lower...
12 December 2014 5,883 7 View
Although I am fully aware that different primer designing software are using different algorithms in their work, still they should give the similar primer pair combinations or not? Also, when some...
08 August 2014 10,054 1 View
SYBR Green Real Time PCR
08 August 2014 7,433 5 View
I run real time sybr green pcr (rat hippocampus) and I came accros a primer dimer situation. I use 42.5 nM primers in final concentration. I am getting PDs in whole range of concentrations, from...
07 July 2014 909 4 View
If you increase annealing temperature, let say from 60 to 63 degrees, will Ct values from the same cDNA concentrations be changed?
07 July 2014 951 5 View
Advantages/disadvantages, troubleshooting when doing real time PCR?
04 April 2014 4,783 10 View
What is the best/optimal way to freeze the rat brain - liquid nitrogen, CO2, isoamyl alcohol? When you take out brain from 30% sucrose, what is the next step?
12 December 2013 5,535 7 View
What are advantages, troubleshooting's solely and/or in comparison with applied qPCR SYBR Green Master Mix?
11 November 2013 2,541 0 View
What is the start and final concentration of you primers in master mix reaction? Also, in cycling stage what are the optimal temperature and the time for sybr green qPCR reaction?
10 October 2013 3,939 6 View
What is the best and user-friendly software for designing and analyzing primers?
10 October 2013 4,831 7 View
Has anyone worked Real Time PCR with cDNA previously transcribed from RNA that had a low 260/230 ratio, around 1? How does this affect the gene expression in Real Time PCR analysis ?
10 October 2013 3,442 12 View
What is the best method (UPLC, elisa assay, etc) and protocol for doing that, and could you share you experiences, troubleshooting etc...
09 September 2013 3,546 5 View
