What is the time schedule for performing the behavioral tests after ICV application of DEX in mice? How to perform FST: in two sessions or in one, when to do sucrose and what is the time table for this test, adjusting to bottles and etc? Some troubleshooting and advice.
I have experience with FST and I can give you advice on that matter. The most important is the water temperature, where if it is different between the animals/groups (even in 1-2 oC) the behavior (duration of swim) can be different. Since the temperature has significant impact on swimming duration.
Yes, thank you Nikola. I realize that water T can be a problem. I have an experience with behavioral tests but this is a little complexed design because of time points of ICV application of DEX and DEX/BSA so I am not familiar to time schedule which should comply.