Has anyone performed novel object recognition and fear conditioning in the same animals?

When testing memory and learning, did anyone combine these tests in the same set of animals? What could be the dynamics in performing these test? What could be pros/cons for doing this kind of...

01 February 2018 483 0 View

Transport of isolated RNA?

After isolated of total RNA from blood lymphocytes what is the best way of transporting the RNA? After dissolving in DEPT-treated water what is the optimal way? Should the samples be on ice or what?

04 May 2016 6,826 4 View

How to do behavioral tests?

What is the time schedule for performing the behavioral tests after ICV application of DEX in mice? How to perform FST: in two sessions or in one, when to do sucrose and what is the time table for...

03 April 2016 382 2 View

Any suggestion of a whole cell western blot protocol from rat testis?

Dear, Anyone has/tried some protocol for extraction of whole cell from rat testis for western blot? Which buffer you used, troubleshooting and similar things.

11 December 2015 786 5 View

How doI dissolve DEX-BSA for intracerebroventricular injections?

09 October 2015 4,774 0 View

Noldus EthoVision Xt multiple footshocks setup?

How to setup multiple foot shocks in Noldus EthoVision XT software? I need to adjust three footshocks in 1,58, 2,28 and 2,58 second from the beging of conditioning! Fs last for 2 seconds?

09 October 2015 8,044 0 View

Freezing behavior statistics?

07 August 2015 2,021 4 View

Does DNase treatment affect the concentration and purity of isolated RNA?

Namely, in our lab when we measured concentration of RNA sample after and without DNAse treatment, we got surprising results. RNA concentration of sample after DNAse treatment was 5 fold lower...

11 December 2014 5,813 7 View

No title

SYBR Green Real Time PCR

07 August 2014 7,368 5 View

Can anyone explain the differences between programs for designing primers?

Although I am fully aware that different primer designing software are using different algorithms in their work, still they should give the similar primer pair combinations or not? Also, when some...

07 August 2014 9,984 1 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

No title

02 March 2021 3,060 3 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View