Is it just saline or for example CSF or is it combination of DMSO and CSF for instance?
Anyone had a problem regarding the infusion of DEX-BSA?
When testing memory and learning, did anyone combine these tests in the same set of animals? What could be the dynamics in performing these test? What could be pros/cons for doing this kind of...
01 February 2018 562 0 View
After isolated of total RNA from blood lymphocytes what is the best way of transporting the RNA? After dissolving in DEPT-treated water what is the optimal way? Should the samples be on ice or what?
04 May 2016 6,893 4 View
We know that p11 transports the 5-HT1B receptor from the cytoplasm to the membrane and increases density of this receptor on postsynaptic membrane and therefore upregulates serotonergic...
04 May 2016 3,382 0 View
What is the time schedule for performing the behavioral tests after ICV application of DEX in mice? How to perform FST: in two sessions or in one, when to do sucrose and what is the time table for...
03 April 2016 460 2 View
Dear, Anyone has/tried some protocol for extraction of whole cell from rat testis for western blot? Which buffer you used, troubleshooting and similar things.
11 December 2015 869 5 View
How to setup multiple foot shocks in Noldus EthoVision XT software? I need to adjust three footshocks in 1,58, 2,28 and 2,58 second from the beging of conditioning! Fs last for 2 seconds?
09 October 2015 8,131 0 View
When you have WT & KO mice and you do a fear extinction experiment in 7 days period, which statistics is appropriate to use to differences between genotype and inter-group differences between...
07 August 2015 2,125 4 View
Namely, in our lab when we measured concentration of RNA sample after and without DNAse treatment, we got surprising results. RNA concentration of sample after DNAse treatment was 5 fold lower...
11 December 2014 5,908 7 View
SYBR Green Real Time PCR
07 August 2014 7,461 5 View
Although I am fully aware that different primer designing software are using different algorithms in their work, still they should give the similar primer pair combinations or not? Also, when some...
07 August 2014 10,077 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I am editing a book for Springer Nature on recent advancements and future trends in biomaterials for orthopedic and trauma applications, with a publication date slated for early next year. If you...
29 July 2024 5,015 9 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...
19 July 2024 8,522 0 View
I have conducted virtual screening using Schrödinger on a database of 17,000 molecules. Unfortunately, I cannot use the system with the Schrödinger license at the moment. I am trying to find a way...
18 July 2024 2,881 4 View