Has anyone performed novel object recognition and fear conditioning in the same animals?

When testing memory and learning, did anyone combine these tests in the same set of animals? What could be the dynamics in performing these test? What could be pros/cons for doing this kind of...

01 February 2018 483 0 View

Transport of isolated RNA?

After isolated of total RNA from blood lymphocytes what is the best way of transporting the RNA? After dissolving in DEPT-treated water what is the optimal way? Should the samples be on ice or what?

04 May 2016 6,826 4 View

Connection between expression of 5ht1a and p11 gene upon stress and AD?

04 May 2016 3,312 0 View

How to do behavioral tests?

What is the time schedule for performing the behavioral tests after ICV application of DEX in mice? How to perform FST: in two sessions or in one, when to do sucrose and what is the time table for...

03 April 2016 382 2 View

Any suggestion of a whole cell western blot protocol from rat testis?

Dear, Anyone has/tried some protocol for extraction of whole cell from rat testis for western blot? Which buffer you used, troubleshooting and similar things.

11 December 2015 786 5 View

Noldus EthoVision Xt multiple footshocks setup?

How to setup multiple foot shocks in Noldus EthoVision XT software? I need to adjust three footshocks in 1,58, 2,28 and 2,58 second from the beging of conditioning! Fs last for 2 seconds?

09 October 2015 8,044 0 View

Freezing behavior statistics?

07 August 2015 2,021 4 View

Does DNase treatment affect the concentration and purity of isolated RNA?

Namely, in our lab when we measured concentration of RNA sample after and without DNAse treatment, we got surprising results. RNA concentration of sample after DNAse treatment was 5 fold lower...

11 December 2014 5,813 7 View

No title

SYBR Green Real Time PCR

07 August 2014 7,368 5 View

Can anyone explain the differences between programs for designing primers?

Although I am fully aware that different primer designing software are using different algorithms in their work, still they should give the similar primer pair combinations or not? Also, when some...

07 August 2014 9,984 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View