Can forward and reverse sequences of PCR products be entirely different from each other?

Dear All, The forward and reverse sequences obtained from the PCR products are not identical at all. Can I still make the contigs for doing BLASTn? Individually both sequences are showing the...

22 December 2020 5,280 5 View

Any simplified protocol or methods of estimation of cellulose, hemicellulose and lignin content in a biomass?

Dear all, Kindly suggest me a simplified/easy method of calculation of cellulose, hemicellulose and lignin content of agricultural biomass along with the relevant reference. We have used the NREL...

02 December 2020 4,362 3 View

Complete protocol for Fungal Genomic DNA isolation?

Dear all, I am looking for recommendations for an efficient protocol for the extraction of Genomic DNA from several fungal cultures. I have most of the chemicals and equipment used in the process,...

20 September 2020 7,906 7 View

What are the different techniques to characterize chemical crystals?

Hello researchers, I would like to know the various techniques/methods which are used to characterize the crystals of a known substance (xylitol) and to check the purity as well as to quantify the...

28 May 2019 3,242 5 View

Please help me in identifying the following species of fly?

Hello researchers, I have attached some photos of a single fly. Please tell me the species of this fly. I shall be highly obliged. Best regards Dr. Sidana

21 March 2017 8,517 7 View

Is there any server/site to predict active sites of drug target enzymes?

24 February 2015 8,874 4 View

How do I add a feature annotation when submitting a sequence in Genbank?

I am trying to submit a gene sequence in Genbank from last 2 months, but they always say something like this.. Complete feature annotation has not been included for some or all of the sequence(s)...

26 January 2015 9,751 4 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View