Dear all,
I inoculated a 200 ml minimal medium with 50g/L glucose with an unknown yeast (shown in figures).
After an incubation of 72 hours, at 180 RPM and 28 C, the broth containing the yeast turned very viscous, slippery liquid. In HPLC, using H column with 5mM Sulphuric Acid as mobile phase and RID as well as UV detector, nothing was detected.
The colour of the yeast is not cream or white, but its like cantaloupe colour.
Any type of help in identifying this yeast or the viscous product shall be highly appreciated.
Best Regards
Arush
Dear Arush ,
Just do ITS sequencing and do blast to find out the which yeast it is.
thank you..
Dear Arush,
Indeed I can only make the same advice to use molecular identification. D1D2 region sequencing might also give you a robust identification. But in addition, you may have some preliminary indications using ITS amplification + RFLP (see Zarzoso, International Journal of Systematic Bacteriology (1999), 49, 329–33).
Regards.
JL
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