Elfadil Abass

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Questions related from Elfadil Abass

Does anyone know of a protocol to isolate hybridomas from peritoneal mouse cavity?

I want to generate polyclonal sera in mice by injecting hybridomas in peritoneal cavity of mice. Could anybody recommend a simple protocol to isolate the cells from peritoneal cavity once...

24 August 2014 190 1 View

What could be the reason protein is expressed in an uninduced E. coli culture as well?

I am expressing a Leishmania protein of about 35kDa in E. coli. My problem is that I always see the same band in uninduced bacteria control lysate which is quite similar to the induced culture...

28 February 2014 5,536 46 View

Why visceral leishmaniasis patients in East Africa do have low levels of antibodies to rK39?

It is well know that VL patgients in East Africa do have low levels of antibodies to the recombinant protein K39. An explanation for that is still unknown! What is the most likely to be the...

01 January 1970 6,295 3 View

What test you recommend for diagnosis visceral leishmaniasis at field endemic areas?

Sensitivity vs. practicability! If you are to choose or recommend a test to be use for diagnosis of visceral leishmaniasis in endemic field areas, do you recommend the Direct Agglutination Test...

01 January 1970 7,866 3 View

Has anyone compared antigenicity between CL and VL parasites?

I’m exploring why serology underperforms in cutaneous leishmaniasis (CL) versus visceral leishmaniasis (VL). Does anyone have published studies or personal experience comparing...

01 January 1970 2,022 1 View

Cow's milk consumption and cancer risk

Cow's milk consumption has a complex relationship with cancer risk. Potential risk factors include hormones like IGF-1 and estrogens, which may encourage cancer cell growth. Considering this, does...

01 January 1970 8,454 2 View