Can forward and reverse sequences of PCR products be entirely different from each other?

Dear All, The forward and reverse sequences obtained from the PCR products are not identical at all. Can I still make the contigs for doing BLASTn? Individually both sequences are showing the...

22 December 2020 5,280 5 View

Any simplified protocol or methods of estimation of cellulose, hemicellulose and lignin content in a biomass?

Dear all, Kindly suggest me a simplified/easy method of calculation of cellulose, hemicellulose and lignin content of agricultural biomass along with the relevant reference. We have used the NREL...

02 December 2020 4,362 3 View

Complete protocol for Fungal Genomic DNA isolation?

Dear all, I am looking for recommendations for an efficient protocol for the extraction of Genomic DNA from several fungal cultures. I have most of the chemicals and equipment used in the process,...

20 September 2020 7,906 7 View

What are the different techniques to characterize chemical crystals?

Hello researchers, I would like to know the various techniques/methods which are used to characterize the crystals of a known substance (xylitol) and to check the purity as well as to quantify the...

28 May 2019 3,242 5 View

Please help me in identifying the following species of fly?

Hello researchers, I have attached some photos of a single fly. Please tell me the species of this fly. I shall be highly obliged. Best regards Dr. Sidana

21 March 2017 8,517 7 View

Is there any server/site to predict active sites of drug target enzymes?

24 February 2015 8,874 4 View

Can someone help me with sequence submission protocol?

Can anybody help me in submitting 8 Leishmania donovani partial gene sequences in genbank (ncbi)? I have eight sequences with small differences. The promoter region of the ldmdr1 gene of L....

27 January 2015 4,893 2 View

How do I add a feature annotation when submitting a sequence in Genbank?

I am trying to submit a gene sequence in Genbank from last 2 months, but they always say something like this.. Complete feature annotation has not been included for some or all of the sequence(s)...

26 January 2015 9,751 4 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

How to improve and implement WBAN MAC and Radio modules under Castalia 3.2 ?

Hello researchers, Hello Castalia3.2 researchers I am a beginner with Castalia, I work on optimization of RFID systems used in wireless body networks. I simulated a WBAN with 10 nodes and one...

28 February 2021 400 1 View