22 Questions 16 Answers 0 Followers
Questions related from Arushdeep Sidana
Dear All, The forward and reverse sequences obtained from the PCR products are not identical at all. Can I still make the contigs for doing BLASTn? Individually both sequences are showing the...
23 December 2020 5,470 5 View
Dear all, Kindly suggest me a simplified/easy method of calculation of cellulose, hemicellulose and lignin content of agricultural biomass along with the relevant reference. We have used the NREL...
03 December 2020 4,563 3 View
Dear all, I am looking for recommendations for an efficient protocol for the extraction of Genomic DNA from several fungal cultures. I have most of the chemicals and equipment used in the process,...
21 September 2020 8,033 7 View
Dear all, I inoculated a 200 ml minimal medium with 50g/L glucose with an unknown yeast (shown in figures). After an incubation of 72 hours, at 180 RPM and 28 C, the broth containing the yeast...
07 August 2020 6,515 4 View
Dear all, Please share the complete method of washing Hi-plex Ca (duo) HPLC column. Solvents used Time for each solvent Any other instructions Thank you
27 June 2020 396 0 View
Dear all, Please help me identifying this fungal species. It has cream to white raised colonies with cheesy appearance.
23 June 2020 8,405 4 View
Hello researchers, I would like to know the various techniques/methods which are used to characterize the crystals of a known substance (xylitol) and to check the purity as well as to quantify the...
29 May 2019 3,364 5 View
Hello all, I had prepared xylitol crystals by yeast fermentation. The white crystals on storage at room temperature turns to light brown in color. I would like to know the reason of this...
29 May 2019 3,368 4 View
Hello researchers, I have attached some photos of a single fly. Please tell me the species of this fly. I shall be highly obliged. Best regards Dr. Sidana
22 March 2017 8,625 7 View
Rules: No more than 5 hydrogen bond donors (the total number of nitrogen–hydrogen and oxygen–hydrogen bonds)No more than 10 hydrogen bond acceptors (all nitrogen or oxygen atoms)A molecular mass...
10 January 2016 3,951 12 View
Dear readers, I am working in a virology lab with all the latest instruments/equipments and I want to write a project on "Dengue virus in India" Please suggest me which type of work can be done on...
11 December 2015 8,682 5 View
How much moisture content (%) is acceptable for the plant powder used for Soxhlet extraction??
22 April 2015 7,385 7 View
How to find the active site(s) in a drug target enzyme for molecular docking? I want to predict possible or confirmed active sites in the following enzymes Adenylosuccinate lyase PDB (4MX2) N-...
25 February 2015 8,985 4 View
According to the WHO report, Cutaneous Leishmaniasis is caused by L. infantum, L. tropica, L. major, L. aethiopica and L. donovani. But L. donovani usually cause Visceral Leishmaniasis. The most...
19 February 2015 6,504 4 View
I have the whole genomic DNA of 33 leishmania isolates. Those may be of L. donovani or L. tropica. Which region of those DNAa is suitable to amplify for species differentiation? ITS region or some...
19 February 2015 6,703 3 View
I have a gene sequence with first start codon (ATG) beginning at 446th nucleotide. Then what is the use of first 445 nucleotides? Do those nucleotides play any role in the functioning/expression...
15 February 2015 7,240 4 View
Kindly tell me how to find promoter in a gene sequence? I am trying to find the promoter region in ldmdr1 gene of Leishmania donovani. The gene sequence is in the following...
04 February 2015 744 1 View
Can anybody help me in submitting 8 Leishmania donovani partial gene sequences in genbank (ncbi)? I have eight sequences with small differences. The promoter region of the ldmdr1 gene of L....
28 January 2015 5,015 2 View
I am trying to submit a gene sequence in Genbank from last 2 months, but they always say something like this.. Complete feature annotation has not been included for some or all of the sequence(s)...
27 January 2015 9,864 4 View
Read 25 answers by scientists with 1 recommendation from their colleagues to the question asked by Arushdeep Sidana on Jan 24, 2015
24 January 2015 1,288 22 View
What is an outlier or out group in phylogenetic analysis. I am constructing a phylogenetic tree of Leishmania parasite, for which I need to choose an outlier. How can I do that?
24 January 2015 8,126 2 View
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27 August 2014 1,009 5 View
