Hi all ,
I want to do an osteogenic differentiation from MSC , so I must feed my cells with osteogenic media to induct the differentiation. I have one question: is my work correct if first I prepare all the stock of osteogenic component alone and then I mix them in one tube and stock them... and when I want to feed the cells ...I take a small amount from the mix and I add media and feed my cells...
Hope someone help us
Best regards
Hi monyer ,
The link doesnt working please you can correct it to me ...thx in advance
I prepare and stock each component alone (at the recommended temperature) and mix them "ex tempore".
Go ahead. If you are storing the stock at freezing temperature make sure to aliquot and store. For example if you need to add 100ul of the component stock to 50 ml of media , aliquot the stock in 50ul and store them . This step is particularly important as you can avoid multiple free-thawing of the stock.
Dear all !
I tried to prepare the osteogenic medium every week so it works very very well .
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