Which is the most efficient method to obtain cell lysate of cultured rat BMSCs for ALP Assay?

I wish to measure  ALP activity in cultured rBMSC. I found few methods to obtain cell lysate using RIPA.  I) scrape out the cells in PBS, pellet the cells and treat in RIPA II) Trypsinise the...

30 June 2017 4,330 8 View