6 Questions 16 Answers 0 Followers
Questions related from Linda Scavo
hello all , to evaluated the adipogenic differentiation , we use oil red o staining , i use 0.3 % or 0.5 % from this solution best regards
12 May 2017 6,844 6 View
I need to quantify the intensity of alizarin red staining / mineralized nodule formation in osteoblasts derived from differentiated MSCs. please there is someone have a protocol to share? thanks...
11 May 2017 6,194 6 View
hello i collect MSC and i want to do immunofluorescene to detect the surface antigen CD -73 AND CD 90 , it is the first time i want to do flow cytometer . someone has a video or a protocol...
08 May 2017 6,021 5 View
hello all i stain MSC differentiated in adipocyte by oil red o , i read that i must use the isopropanol to do quantifiying , please some one can help me by sending the protocol . thx in advance
07 May 2017 5,894 7 View
Hi all , I want to do an osteogenic differentiation from MSC , so I must feed my cells with osteogenic media to induct the differentiation. I have one question: is my work correct if first I...
01 May 2017 3,869 7 View
hi all , i want reconstitute a masse of collagenase type 2 with HBSS , i want a concetration stock 1000 u /ml , how can i reconstitute it , i.e how much masse of collagenase i must take with...
01 December 2016 6,917 2 View