10 Questions 21 Answers 0 Followers
Questions related from Linda Scavo
I'm cultturing Placenta derived MSCs and am repeatedly finding the formation of crystals in my cultures. They're either pyramidal or cuboidal in morphology No-one else in my lab experience of...
27 May 2017 7,176 6 View
hello all !!i found in my cell culture ( placenta derived MSCs ) crystals (Calcium Oxalate) .I am culturing the Pl-MSCs using Alpha-Mem 10%FCS / 1%Pen-strep. I experienced crystals similar...
24 May 2017 1,316 2 View
hello all , to evaluated the adipogenic differentiation , we use oil red o staining , i use 0.3 % or 0.5 % from this solution best regards
12 May 2017 6,884 6 View
I need to quantify the intensity of alizarin red staining / mineralized nodule formation in osteoblasts derived from differentiated MSCs. please there is someone have a protocol to share? thanks...
11 May 2017 6,234 6 View
Hello all !! I am interested in performing flow cytometry on human placenta derived MSC . Does anyone have a cohesive Flow Cytometry staining cell surface marker cd 105 , cd 73 protocol that they...
09 May 2017 2,211 5 View
hello i collect MSC and i want to do immunofluorescene to detect the surface antigen CD -73 AND CD 90 , it is the first time i want to do flow cytometer . someone has a video or a protocol...
08 May 2017 6,062 5 View
hello all i stain MSC differentiated in adipocyte by oil red o , i read that i must use the isopropanol to do quantifiying , please some one can help me by sending the protocol . thx in advance
07 May 2017 5,964 7 View
Hi all , I want to do an osteogenic differentiation from MSC , so I must feed my cells with osteogenic media to induct the differentiation. I have one question: is my work correct if first I...
01 May 2017 3,910 7 View
hi all , i want reconstitute a masse of collagenase type 2 with HBSS , i want a concetration stock 1000 u /ml , how can i reconstitute it , i.e how much masse of collagenase i must take with...
01 December 2016 6,957 2 View
hi all ! how do you get them off the plate surface without compromising there physicalproperties on the cytometer? does anyone use accumax or accutase solution solution to detach MSC ?? if so...
26 October 2016 7,750 3 View