Linda Scavo

6 Questions 16 Answers 0 Followers

Questions related from Linda Scavo

Can someone help me about oil red o staining ?

hello all , to evaluated the adipogenic differentiation , we use oil red o staining , i use 0.3 % or 0.5 % from this solution best regards

12 May 2017 6,843 6 View

Please i need help about quantification of alizarin red ?

I need to quantify the intensity of alizarin red staining / mineralized nodule formation in osteoblasts derived from differentiated MSCs. please there is someone have a protocol to share? thanks...

11 May 2017 6,192 6 View

Can someone help me in flow cyotmeter to stain cell surface marker for MSC ?

hello i collect MSC and i want to do immunofluorescene to detect the surface antigen CD -73 AND CD 90 , it is the first time i want to do flow cytometer . someone has a video or a protocol...

08 May 2017 6,019 5 View

Please i need your help ?

hello all i stain MSC differentiated in adipocyte by oil red o , i read that i must use the isopropanol to do quantifiying , please some one can help me by sending the protocol . thx in advance

07 May 2017 5,892 7 View

Can someone help me?

Hi all , I want to do an osteogenic differentiation from MSC , so I must feed my cells with osteogenic media to induct the differentiation. I have one question: is my work correct if first I...

01 May 2017 3,867 7 View

Can someone help me to calculate the masse of enzyme taken to obtain c=1000 u/ml

hi all , i want reconstitute a masse of collagenase type 2 with HBSS , i want a concetration stock 1000 u /ml , how can i reconstitute it , i.e how much masse of collagenase i must take with...

01 December 2016 6,916 2 View