Linda Scavo

10 Questions 21 Answers 0 Followers

Questions related from Linda Scavo

How do I prevent the formation of crystals in cell culture?

I'm cultturing Placenta derived MSCs and am repeatedly finding the formation of crystals in my cultures. They're either pyramidal or cuboidal in morphology No-one else in my lab experience of...

27 May 2017 7,178 6 View

I need urgently your help , can someone help me to avoid calcium oxalate in my cell ?

hello all !!i found in my cell culture ( placenta derived MSCs ) crystals (Calcium Oxalate) .I am culturing the Pl-MSCs using Alpha-Mem 10%FCS / 1%Pen-strep. I experienced crystals similar...

24 May 2017 1,319 2 View

Can someone help me about oil red o staining ?

hello all , to evaluated the adipogenic differentiation , we use oil red o staining , i use 0.3 % or 0.5 % from this solution best regards

12 May 2017 6,885 6 View

Please i need help about quantification of alizarin red ?

I need to quantify the intensity of alizarin red staining / mineralized nodule formation in osteoblasts derived from differentiated MSCs. please there is someone have a protocol to share? thanks...

11 May 2017 6,236 6 View

Does anyone have a Flow Cytometry Protocol to share?

Hello all !! I am interested in performing flow cytometry on human placenta derived MSC . Does anyone have a cohesive Flow Cytometry staining cell surface marker cd 105 , cd 73 protocol that they...

09 May 2017 2,213 5 View

Can someone help me in flow cyotmeter to stain cell surface marker for MSC ?

hello i collect MSC and i want to do immunofluorescene to detect the surface antigen CD -73 AND CD 90 , it is the first time i want to do flow cytometer . someone has a video or a protocol...

08 May 2017 6,064 5 View

Please i need your help ?

hello all i stain MSC differentiated in adipocyte by oil red o , i read that i must use the isopropanol to do quantifiying , please some one can help me by sending the protocol . thx in advance

07 May 2017 5,966 7 View

Can someone help me?

Hi all , I want to do an osteogenic differentiation from MSC , so I must feed my cells with osteogenic media to induct the differentiation. I have one question: is my work correct if first I...

01 May 2017 3,912 7 View

Can someone help me to calculate the masse of enzyme taken to obtain c=1000 u/ml

hi all , i want reconstitute a masse of collagenase type 2 with HBSS , i want a concetration stock 1000 u /ml , how can i reconstitute it , i.e how much masse of collagenase i must take with...

01 December 2016 6,958 2 View

Do any of you do get the mesenchymal stem cell ?

hi all ! how do you get them off the plate surface without compromising there physicalproperties on the cytometer? does anyone use accumax or accutase solution solution to detach MSC ?? if so...

26 October 2016 7,753 3 View