Experiment or Quasi Experiment Design?

What kind of study design are we talking about? Subjects are randomly assigned to one of three groups. In addition, a moderator and a mediator are collected as continuous variables. Is this an...

15 January 2021 1,809 4 View

What is the best solvent for the extraction of chemical compounds that have an insecticidal effect ?

What is the best solvent for the extraction of chemical compounds that have an insecticidal effect ?

26 November 2020 4,609 15 View

Nvivo - how to extract and analyze hashtag?

Hello, I am using Nivo 12.0 plus. I've a dataset of tweets, unfortunately not extracted by NCapture. How can I extract and analyse hashtag? Is it possibile using auto-code functions? Thank you

19 August 2020 9,532 3 View

What is a scoping review?

In what context should we choose to do a scoping review? How is it different from systematic review, literature review, mapping review and Cochrane review? What are its mandatory or optional...

05 July 2020 8,206 3 View

If I use REVEL score as a meta-predictor for clinical variant classification, what would be a reasonable cut-off to filter disease variants?

Ioannidis et al., 2019, reported the development of the REVEL score (rare exome variant ensemble learner) for redefining pathogenic variant classification, and Tian et al., 2019, reported that...

29 March 2020 2,450 2 View

Is there a method to statistically compare two or more regression coefficients in paired data?

Hello, I have conducted simple linear regression for four time points, always on the same sample. My time points are 10 years apart and now I wish to compare the slopes / betas / regression...

21 March 2020 10,020 9 View

Is the Case Study Method eligible to create theory?

I have been conducting research for PhD Theses using case study as a method, mainly refer to Robert K. Yin works. At the final step of my research, I define some concepts and use the concepts to...

12 February 2020 3,146 10 View

What is the blue-shift in UV-vis?

hello, Im having trouble in extracting information from my uv-vis data. The sample the I analyzed is protein corona. Before that, I already characterize 40 nm of gold nanoparticle and obtain its...

15 May 2018 8,815 7 View

Does someone have neuronal cell cultures of zebrafish?

I plan to use immunohistochemical methods to stain brain sections from adult fish (teleosts, starting with zebrafish). In order to validate the antibodies I would like to test them in a 2D-celll...

10 September 2017 6,280 1 View

Please does anyone knows how to use genome mining in search of genes encoding for particular enzymes?

For instance if I want to search for genes encoding particular enzymes which bioinformatic tool is better and how do I select the best genes to investigate?

14 August 2017 1,967 4 View

Can i quantify protein concentration in sds page?

I have to quantify a mixture of proteins. But I can not quantify its concentration by any protein detection methods as the values fluctuate. Can i quantify the protein concentration by sds page?

22 February 2021 215 2 View

How to convert 2D stack images to 3D to obtain surface area of nucleus?

I have 15 slices of the nucleus at different z-heights from confocal imaging. I would like to use these slices to reconstruct the 3D nucleus and ultimately estimate the surface area of the...

20 February 2021 6,967 1 View

Can ImageJ software be used to quantify collagen from Masson's-Trichrome stained images?

I am after quantifying the blue stained collagen in a Masson's-Trichrome stained skin section from an immune deficient mice and compared the same with its WT control. I am curious to know can...

17 February 2021 384 3 View

Does anyone knows about a really easy but still comprehensive ImageJ tutorial?

I am new to this software. I am in need of analyzing images from ocular ultrasounds (black/white) and find a way to quantify the internal reflectivities from the vitreous cavity in cases of...

16 February 2021 4,238 2 View

How to measure fluorescence intensity of Dapi stained nuclei?

I have tried various methods using imageJ to measure mean fluorescence intensity of dapi stained nuclei but none of them seem to be working. Ive attached an image i am trying to measure the...

15 February 2021 8,637 3 View

Benefit of CTCF (corrected total cell fluorescence)?

03 February 2021 6,302 2 View

How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

How can I analyze the morphology of microglia (ramification, braches, soma, etc.) via Fiji/ImageJ?

Hey everybody, for my project I need to analyze the morphology of microglia, which I have previously stained (IHC) and scanned in a SlideScanner. Specifically, I need the ramification, number and...

31 January 2021 3,855 3 View

How credible is the average size of nanoparticles obtained using SEM image and ImageJ software?

My research work requires synthesizing nanoparticles, characterisation before application. How credible is the average size obtained from ImageJ software using images from SEM or TEM?

30 January 2021 3,838 3 View

How to get the insoluble fraction of PK digestion for mitochondria?

I am trying to check for some proteins if they are located in the inner or outer mitochondria membrane after treating the cells with a neurotoxin. I am using PK to digest the mitochondria, but I...

27 January 2021 5,145 2 View