Can someone explain how to carry out DNA fingerprinting in crop plant without reference genome and no locus information?

Is it possible to develop DNA fingerprinting for varietal identification of crop plants without reference genome and no locus information except the availability of set of DNA markers.

25 January 2021 6,826 4 View

Can someone help me find a way to quantify leaf shape?

I 'am looking for a most simplified formula to quantify shape of leaves mathematically which will help me to categorise plants based on leaf shape.Earlier,I tried with some image processing...

10 December 2020 2,386 5 View

In supervised classification, how polygon training sample works?

If I select polygon samples to train my model, how the value of pixels used? Does the model take DN of each pixel or the average of each object (polygon)?

01 October 2020 4,882 2 View

Can I send leaf samples crushed and powdered in liquid nitrogen for ploidy analysis without icepacks?

I need to send few powdered leaf samples crushed using liquid nitrogen for flow cytometric analysis.Will the DNA get damaged if it's not packed in ice?

19 June 2020 7,259 6 View

Can someone help me with Genome wide association study in crop plants to find marker trait association?

Hi, I have trouble in understanding GWAS research papers (as they are full of statistics... )for finding phenotypic to genotypic relationship in crop plants.Can someone suggest me the basic...

15 January 2020 4,914 2 View

Can anyone help me with Genomic DNA isolation from curcuma longa using ctab method?

The genomic DNA,I isolated from turmeric using ctab .The protocol followed for genomicDNA isolation is: grind turmeric leaves using DNA extraction buffer(100 mM tris PH 8,20 mM EDTA,1.4 M Nacl,2%...

07 November 2019 9,428 10 View

Why stress values of composite girder shows both compression and tension at many nodes near the interface of concrete and steel?

when extracting stress values S33 at mid section of composite girder analysed in ABAQUS, getting both negative and positive values at 5 - 6 nodes nearer to the interface. this indicates like...

06 June 2019 7,901 2 View

Can you please tell why nanoparticles are not soluble in most of the solvent?

I am doing Msc project,so my Msc project I want to ask this question. Nano particles are not soluble in most of the solvent

13 February 2019 4,312 8 View

Why does Uniform Distributions have no outliers?

I was reading about the relation between Kurtosis and Outliers on Wiki and got across a line 'An example of a platykurtic distribution is the uniform distribution, which does not produce outliers'...

02 August 2018 8,845 3 View

Why there is no variation in capacitance with body load in Comsol?

Hi,I am trying to find variation in capacitance between two plates with applied body load. I defined an air box around the plates. Now I'm trying with electromechanics physics for applying body...

13 April 2016 1,935 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How UAV or drone can be beneficial to better estimate crop yield?

As we know, crop yield estimation has been done traditionally with farmer's expert knowledge, deductive sampling in representative areas, and remote sensing with satellite imagery. Farmer's Expert...

01 March 2021 3,468 3 View

Analysis of CRD two factor using R studio ?

Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...

28 February 2021 8,189 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

Where i will get the Desmodium and Napier grass seeds?

26 February 2021 975 4 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

SSR primers are species specific or we can use it for any plant species ?

Hi, any one please guide me; SSR primers are species specific or trait specific. i have few primer which was reported in articles for wheat yield can i use these similar primer for lentil yield...

20 February 2021 315 4 View

What are the LAB methodologies (procedures, equipment etc) for CO2 enrichment experiments to study crop responses?

I am doing a proposal for a research entitled 'GRAIN YIELD AND QUALITY RESPONSES OF WHEAT GENOTYPES UNDER CO2 ENRICHMENT AND EXTREME EVENTS' I will be using climate chamber and I would like to...

18 February 2021 6,494 4 View

Antibody amino acid sequence database to simulation?

Hello, I need your help. I am looking for a database of antibody amino acid sequences for later simulation in a program in order to analyze their structure. The antibodies I need to test...

18 February 2021 371 4 View