Review and cite CROP SCIENCE protocol, troubleshooting and other methodology information | Contact experts in CROP SCIENCE to get answers
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Good day! I am currently testing for the antioxidant activity of my extract via the hydrogen peroxide scavenging assay. I have already done the experiment in six trials using 30% hydrogen...
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To create a cell line overexpressing a specific microRNA, I'm planning to synthesize and clone the shRNA sequence into a vector. Would it be sufficient to add only the termination sequence (TTTTT)...
28 May 2024 4,030 0 View
the query centers on determining the most efficient and precise latest technology available in the market, capable of swiftly analyzing complex mixtures while maintaining a high level of...
27 May 2024 2,725 6 View
While recording electrical activity from the prefrontal cortex of primates it has been found that a 1-9 Hz burst of activity (i.e., theta activity) occurs immediately before a perceptual...
25 May 2024 5,907 0 View
Need a simple and small scale preparation methods
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I have a paper that was written in Microsoft Word using a journal template, and I would like to convert it to a LaTeX template. I'm wondering if there's a way to automate this conversion process,...
21 May 2024 7,196 2 View
Climate change directly impacts agricultural productivity in various ways, affecting crop yields, livestock health, and the overall sustainability of farming practices. How we can characterized...
19 May 2024 4,246 6 View
Dear All, We have downloaded sentinel 2 data for measuring Chlorophyll Concentration in seawater. We have processed sentinel 2 data in SNAP software and also applied standard...
16 May 2024 3,163 1 View
Hey everyone! I have a few peptide sequences synthesized using SPPS and all of them are in the M.wt range of 6 to 8kDa. I've done MALDI MS and found out that I do have the desired peptide peak but...
14 May 2024 5,778 2 View
I want to measure MDA concentrations using the TBARS assay in in vitro cell lines. Currently, I am following the protocol as described by (Aguilar et al., 2022) in their paper "Evaluation of...
13 May 2024 7,357 0 View
I have 0.2ml of essential oil X weighing about 200mg, I wanted to diluted it to a final volume of 5 ml, calculate its concentration in mg/ml ? Thank you.
11 May 2024 5,760 2 View
Please can you answer my question I have an extract that I dried and made different concentrations to calculate the percentage of phenolic compounds using Gallic acid as a standard using the Folin...
08 May 2024 5,424 3 View
I bought a lipid peroxidation kit from Sigma and I want to get kits to measure nitrates, superoxide dismutase, catalase, and glutathione. Each one of those kits comes with a different lysis...
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impact scenario of fertilizers consumption pre and post scheme of soil heath card
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How do we balance environmental protection and economic progress and how can we manage our environment for sustainable development?
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Normally, after extracting a plant with solvents such as ethanol, methanol, and water, the solvent is removed, and the extracts are pulverized. A stock solution is then prepared from the powdered...
02 May 2024 2,282 9 View
Hi, I'm currently trying to generate a one-vector plasmid system for a knockout using CRISPR/Cas9. I'm using lentiCRISPRv2 (ampicillin-resistant) and following the GeCKO Target Guide Sequence...
30 April 2024 3,520 0 View
Dear Researchers, I would like to start working with Caco-2. I have seen in articles that different media (e.g. MEM or DMEM with various suplementation) are used to culture Caco-2. Which is the...
30 April 2024 4,805 2 View
I tried to inhibit extracellular microvesicles(MVs) secreted from cells, through using Rock inhibitor(Y27632), calpeptin and several siRNAs(siCDC42, siTSG101, siARF1), but failed to decrease MVs....
24 April 2024 7,229 0 View
Hello, I am looking for a series of over 30 molecules derived from the cannabis plant with a specific biological activity and the IC50 values of all the molecules. Could you help me find this...
23 April 2024 9,330 0 View
I am currently preparing research methods for my thesis. My sample is an exosome from Adipose. However, I had difficulty identifying what types of proteins were in it. Can anyone help me?...
20 April 2024 4,391 2 View
But the urea should not be converted as liquid during synthesis
20 April 2024 7,805 4 View
I need a research topic related to recent trends in GST
17 April 2024 1,677 0 View
Additionally, we aim to predict whether the chromatin is in a condensed or relaxed state!!
16 April 2024 7,311 3 View