Review and cite CROP SCIENCE protocol, troubleshooting and other methodology information | Contact experts in CROP SCIENCE to get answers
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Questions related to Crop Science
Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...
19 June 2024 2,814 4 View
Is there a protocol to establish diabetic conditions in pre-osteoblasts cell culture?
19 June 2024 9,318 2 View
I'been seeing those white spots at the end of my wet-transfer lately. Since the Ponceu Red doesn't stain I guess there isn't any protein. Since it's under the 42kDa mark It isn't a problem since I...
19 June 2024 1,745 0 View
A question to see who can answer it. If the red and infrared bands of the satellites are...
17 June 2024 2,266 1 View
My amino acid condensation reaction (the reagents do not have protection groups) utilizes K2CO3 as a base. K2CO3, the resulting peptide product and unreacted amino acids all have decent solubility...
17 June 2024 7,733 3 View
Hi all, you know this lncRNA has 12 exons and 50 isoforms that just have similar sequences in exon 12. Coud someone recommend me primers for this exon with no need of probes?
16 June 2024 8,069 0 View
Hi, I am working on the flourescence sensing of heavy metal ions in aqeous media using chalcogenide nanoparticles. If I dope a metal ion into this nanoparticles by reducing the moles of already...
12 June 2024 5,581 2 View
Mammals (from rodents to primates) can detect and therefore become aware of visual stimuli exhibiting luminance-contrast levels as low as 1 to 2% (Schiller and Tehovnik 2015; Histed et al. 2012)....
11 June 2024 4,279 1 View
I am currently working on a study using RAW 264.7 cells. I am polarizing the cells to an M1 phenotype. I am then applying an anti-inflammatory compound 24 hours later. These cultures are being...
10 June 2024 889 1 View
Good day! I am currently testing for the antioxidant activity of my extract via the hydrogen peroxide scavenging assay. I have already done the experiment in six trials using 30% hydrogen...
28 May 2024 4,690 3 View
To create a cell line overexpressing a specific microRNA, I'm planning to synthesize and clone the shRNA sequence into a vector. Would it be sufficient to add only the termination sequence (TTTTT)...
28 May 2024 4,217 0 View
the query centers on determining the most efficient and precise latest technology available in the market, capable of swiftly analyzing complex mixtures while maintaining a high level of...
27 May 2024 2,909 6 View
While recording electrical activity from the prefrontal cortex of primates it has been found that a 1-9 Hz burst of activity (i.e., theta activity) occurs immediately before a perceptual...
25 May 2024 6,109 0 View
Need a simple and small scale preparation methods
24 May 2024 3,853 0 View
I have a paper that was written in Microsoft Word using a journal template, and I would like to convert it to a LaTeX template. I'm wondering if there's a way to automate this conversion process,...
21 May 2024 7,406 2 View
Climate change directly impacts agricultural productivity in various ways, affecting crop yields, livestock health, and the overall sustainability of farming practices. How we can characterized...
19 May 2024 4,427 6 View
In the mid-1980's at the University of Toronto, John Eccles delivered a lecture on consciousness. Eccles, being a dualist, declared that the interface to consciousness is in the supplementary...
17 May 2024 3,211 1 View
Dear All, We have downloaded sentinel 2 data for measuring Chlorophyll Concentration in seawater. We have processed sentinel 2 data in SNAP software and also applied standard...
16 May 2024 3,351 1 View
Hey everyone! I have a few peptide sequences synthesized using SPPS and all of them are in the M.wt range of 6 to 8kDa. I've done MALDI MS and found out that I do have the desired peptide peak but...
14 May 2024 5,978 2 View
I want to measure MDA concentrations using the TBARS assay in in vitro cell lines. Currently, I am following the protocol as described by (Aguilar et al., 2022) in their paper "Evaluation of...
13 May 2024 7,551 0 View
I have 0.2ml of essential oil X weighing about 200mg, I wanted to diluted it to a final volume of 5 ml, calculate its concentration in mg/ml ? Thank you.
11 May 2024 5,948 2 View
Please can you answer my question I have an extract that I dried and made different concentrations to calculate the percentage of phenolic compounds using Gallic acid as a standard using the Folin...
08 May 2024 5,629 3 View
I bought a lipid peroxidation kit from Sigma and I want to get kits to measure nitrates, superoxide dismutase, catalase, and glutathione. Each one of those kits comes with a different lysis...
08 May 2024 4,594 2 View
impact scenario of fertilizers consumption pre and post scheme of soil heath card
07 May 2024 5,231 4 View