When I received purchased cell lines from ATCC, do I still need to verify the cells? Some say no need but some say I have to. Not sure which one I should follow.
I wonder if the publication of these following papers had not impacted on laboratory practice.
Breitkopf SB, Yuan M, Pihan GA, Asara JM
(2012) Detection of a rare BCR-ABL tyrosine kinase fusion protein in H929 multiple myeloma cells using immunoprecipitation (IP)-tandem mass spectrometry (MS/MS) Proc Natl Acad Sci USA 109(40):16190–16195
And
MacLeod RAF, Nagel S, Dirks W, Drexler HG
(2012) BCR–ABL1 expression in multiple myeloma cells: A case of mistaken identity? Proc Natl Acad Sci USA 110:E270–E271.
The work was usually done to verify cell origin is tracing "gene expression profile" by using real time qPCR or expression micro-array methods . This commonly is used in the cases there is a claim to establishment of "new cell line model". But expression profiling is very expensive work and I don't think affordable for your case.
Some journals ask for the authentication of cell lines used in research but usually if these cell lines were not bought from reliable source (eg., you got them from some lab who is not sure where they got it) or bought more than 3-5 years from reliable source. As ATCC is a very reliable source and You have the documentation when You have bought these cells, then You have no problems for the next few years with authentication. And usually they ask only for authentication of human cell lines.
Hi Abu,
ATCC is normally reliable but they do not authenticate all their lines. In the >50 cell lines that we have received from vendors (expanded 2 passages and then tested), only 2% were incorrect. We found out this was due to labeling error from vendor. If you need to have your cell lines authenticated (human or mouse), please have a look at this detailed website: http://www.iqbiosciences.com/bioservices/testing-services/cell-line-authentication
Best Regards,
Omar
Thanks Omar,
It occurred to me that in various research endeavor, some form of local standardization is expected; i.e calibrating a microphone, standardization of normal values or characterization of a measurement method. This is done in the context of the researchers local setup. I suppose based on the response I have received; in the context of cell lines, if purchase from a reputable source like ATCC, the general statement is to take as it is.
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