Babak Nami

5 Questions 82 Answers 0 Followers

Questions related from Babak Nami

How can i determine whether my protein is cationic or anionic?

Hello all, I have a 61 kDa protein with an isoelectric point of 8.24. I think it is slightly cationic. what is its charge? Thanks

07 July 2016 7,876 12 View

Does bacteriophage (T2, T4,..) genome works in human cells?

I want to transfect phage T2 genome into HEK293 cells in order to produce phage particles and cell lysis. But I don't know whether the genes will work or not. What is phage biology in mammalian cells?

10 October 2013 997 2 View

Why do circular DNAs (plasmid, BAC, ...) make a smear during running in agarose gel?

Circular DNA makes a smear when running in agarose gel, but the digested linear form doesn't. I am not talking about covalently or supercoiled bonded.

07 July 2013 7,543 11 View

Can anybody provide a list of common "fusion genes" that are seen in tumor cells?

I know Bcr-Abl fusion gene that is used as an cytogenetics marker for ALL. Thank You...

07 July 2013 3,630 11 View

What is this kind of morphology?

These are AGS cells (Gastric cancer cell line) after incubation in a low pH stale RPMI + 1% L-glutamine +10% FBS + 2% antibiotic for 10 days without medium refresh. When I check the cells most...

06 June 2013 5,410 8 View