Can soemone help me with 4T1 mammospheres aggregation problem?

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Ranjani Rajapaksa

Please see these papers. They may help you out.

Cancer Letters vol.323(1) 2012 20-28.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3672101/pdf/pone.0064388.pdf

Good Luck!

Go J Yoshida

4T1 murine breast cancer cells show heterogeneous expression pattern of CD44 (especially CD44 variant 8-10), so that mammosphere formation assay allows you to enrich cancer stem-like cells in the single cell line.