Hello everyone,
Is there any solution to reduce considerably the 4T1 mammospheres aggregation.Is there any other agent that can replace methyl cellulose?
Please see these papers. They may help you out.
Cancer Letters vol.323(1) 2012 20-28.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3672101/pdf/pone.0064388.pdf
Good Luck!
4T1 murine breast cancer cells show heterogeneous expression pattern of CD44 (especially CD44 variant 8-10), so that mammosphere formation assay allows you to enrich cancer stem-like cells in the single cell line.
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