I made liposomes using Ethanol injection method, I try DPPC (2.5, 3.4, 5 mM) in acetate buffer.

I fixed  the volume ratio of Etoh : buffer (pH 5.3) to 1:20

but i think No Liposome were formed (20nm), I don't know where is the problem

if not how much cholesterol can i add to make it?

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