I made liposomes using Ethanol injection method, I try DPPC (2.5, 3.4, 5 mM) in acetate buffer.
I fixed the volume ratio of Etoh : buffer (pH 5.3) to 1:20
but i think No Liposome were formed (20nm), I don't know where is the problem
if not how much cholesterol can i add to make it?
I am so sorry that I can't reply you in time. I think liposome could be prepared with DPPC and cholesterol . Please note the hydration temperature
I am so sorry that I can't reply you in time. I think liposome could be prepared with DPPC and cholesterol . Please note the hydration temperature
I am so sorry that I can't reply you in time. I think liposome could be prepared with DPPC and cholesterol . Please note the hydration temperature
KEEP LIPID CONCENTRATION 0.5 MM AND TRY ONCE IN PHOSPHATE BUFFER using the same method KEEPING pH 7.4 as in acidic condition liposome breaks down.
I think no need to add cholesterol to DPPC. But if you want mixed liposome then you can add. Size of liposome in this method can vary from 100 nm to 300 nm.
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