I'm trying to find a method to study the number of active mature synapses of purkinje cells (PCs) in the critical week of cerebellum development. I thought about mesuring AMPA-mediated mEPSC (whole-cell patch clamp). However, I'm not sure if I can compare different ages between P10 and P15 considering that the arborization of PCs is critically different at these ages and I can have space-clamp problems.
Do you think you can help me?