21 January 2019 1 6K Report

in other words, doesn't sodium citrate inhibit growth of Lactic Acid Bacteria?

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How do I make 10 cc of enzyme solution with a concentration of 0.2 units/mL?

Hi We have alpha-amylase enzyme that written 5 units /mg protein on its container. How do I make 10 cc of enzyme solution with a concentration of 0.2 units/mL?

08 July 2024 2,285 2 View

Why MiFish Primers amplify when they're alone but not when they have an overhang sequence (Illumina adapter + tag)?

Good day everyone. I'm working with eDNA Metabarcoding for freshwater fishes in Brazil, I ran tests amplifying positive controls and samples with the primer MiFish-U (Miya et al 2015) alone (no...

11 January 2024 5,109 2 View

How to extract total RNA from chondrogenic micromass?

Hi, I am trying to extract total RNA from chondrogenic micromass after 14days of chondrogenesis. I have used Qiagen RNAesay plus mini kit. It was extremely difficult to dissolve the micromass in...

29 November 2023 2,226 2 View

Can you tell me about the NBT test protocol to assess neutrophil function?

I want to prepare NBT solution, but I don't know how much NBT powder to dissolve in PBS. Also, I don't have a BSA, can I do the NBT test without a BSA?

14 November 2023 5,724 1 View

What are available methods for modeling mutual negotiation between players in a coalition game?

In the literature, mutual negotiation hasn't been considered in a cooperative game.

04 June 2023 1,217 0 View

Kindly, suggest me some available methods for modeling distributed decision-making of participants?

As we know, asynchronous energy pricing such as double auction and negotiation allows the execution of several bilateral contracts between prosumers and consumers. In the case of a double auction...

04 June 2023 6,279 0 View

Does anyone have experience working with Wharton's jelly and menstrual blood mesenchymal stem cells (WJ-MSCs and Mens-MSCs)?

I am culturing menstrual blood msc and Wharton's jelly which do not grow well and die in part 4 and 5, especially Wharton's jelly does not grow well. While all the conditions are the same as...

03 March 2023 6,933 2 View

Measurements of sarcomere alignment in thick tissues?

Hello, cardiac tissue engineers! I am a cardiac tissue graduate student, I have fabricated a 50-um cardiac tissue with cardiomyocytes, and want to characterize it, I fixed and stained my tissue...

23 February 2023 3,693 0 View

In SNRS cases What kind of biopsy results lead the physician to Genetic testing directly?

There are currently no guidelines for the use of genetic testing for SRNS in clinical practice. In SNRS cases What kind of biopsy results lead the physician to Genetic testing directly?

05 July 2022 7,976 0 View

Without using NMR technique how can we check exopolysaccharides are completely hydrolyzed?

I used a 2 M trifluoroacetic acid solution and hydrolyzed the EPS at 100°C for 2 h. How can I distinguish this method hydrolyzed the samples completely? I don't have access to NMR

19 May 2022 4,704 4 View

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Can we detect DOC fractions through LC-OCD for a sample containing Sodium Azide?

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What can be done when electronic minimization does not converge during geometry optimization in CASTEP?

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Is it possible for the soil pH to be around 8, but with negligible amounts of carbonates, bicarbonates and exchangeable sodium?

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Nicotiana benthamiana seeds sterilization methods for tissue culturing?

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How to choose the time and acid-base environment for the Clayson Schmidt reaction?

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Are the calibration curves of NO radical scavenging assay asymtotes?

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