Can I use protein with glycerol (added to prevent protein aggregation, and maintain stability during -80 storage) in mice (intraperitoneal) to study anti-inflammatory properties of the protein?
Just a note: For small volumes using molecular weight cut off centrifugal filters should also remove most of the glycerol. Also have you considered dialysis?
Here is a paper on toxicity of intraperitoneal glycerol in mice:
http://www.ncbi.nlm.nih.gov/pubmed/18430056
Hi Komal, I'm curious about the inflammation assay you are going to do. Are you going to inject a inflammation inducing agent along with your anti-inflammatory protein?
IP injections by themselves can cause inflammation.
Thank you all for the answers.
@ Steingrimur Stefansson, yes I am going to inject inflammation inducing agent pre and post protein injection.
@Adam B Shapiro, I have gone through that paper, but the final concentration of glycerol in the protein I will be injecting is 10-15 times less than that used in the paper, so I am not sure of the effect.
Hi Komal, I assume that the you are not studying inflammation in the peritoneum, right?
Anyway, just be aware that IP injections can elicit a inflammatory response that can destroy your protein before it can reach its destination.
Are you going to use a carrier for your protein, like Matrigel or agarose?
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@Steingrimur Stefansson, What I think is, by the time inflammation is generated against my protein, the protein might have done it's job, Correct me if I am wrong.
No, I am not using any carrier.
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