Komal Dolasia

9 Questions 6 Answers 0 Followers

Questions related from Komal Dolasia

How to collect peritoneal lavage from CLP mice for CFU?

Hi, In CLP (cecal ligation and puncture) mice the peritoneal cavity has cecum punctured (the reservoir of microbes), so washing the cavity might lead to leaching of bacteria from cecum, then wont...

10 October 2017 9,091 0 View

What loading control should be used for western blotting if samples are from differentiated and undifferentiated cells?

Hello, I am doing western blotting from Human monocyte derived dendritic cells, differentiated in presence or absence of compound that inhibits the differentiation. I have tried using actin as...

09 September 2017 5,524 3 View

Markers for Mouse Memory B cells?

Which markers can be used to define Mouse Memory B cell, I have got panel of CD19, B220, CD38, CD80,CD273 anitbody based on references, but now I am confused how to define Memory B cells from this...

05 May 2016 8,278 5 View

Minimum time point for checking T reg generation/expansion/induction upon protein injection in mice?

Can I get regulatory T cell generation/expansion/induction in mice with in 12 to 20 hrs of protein IP injection in mice along with infection, Initially I will be checking on the basis of FOXP3....

07 July 2015 4,909 2 View

Can I use primary antibody solution with two antibody classes for Flow cytometry?

I have this antibody solution that contains two antibody classes, IgG1κ and IgG2bκ, Can I use this as primary antibody for flow cytometry? If so then what isotype control should I use, if...

03 March 2015 741 3 View

How can I store samples for western blotting?

Which is the most efficient way to store samples for general western blotting (not phospho) for weeks, as cell pellet at -20°C or in RIPA/lysis buffer after lysis or protein with SDS sample buffer...

01 January 2015 6,850 5 View

Can I use protein containing glycerol for intra-peritoneal injection in mice?

Can I use protein with glycerol (added to prevent protein aggregation, and maintain stability during -80 storage) in mice (intraperitoneal) to study anti-inflammatory properties of the protein?

01 January 2015 6,360 6 View

Should experiments to check the effect of oxidative stress on macrophage be done in the presence or absence of serum?

I want to check the effect of hydrogen peroxide on macrophage, and I am in dilemma whether the experiment has to be done in presence or absence of serum, as in physiological scenario serum will be...

06 June 2014 8,055 3 View

Which is the best method for overexpression of a protein in Mouse macrophage by virus or by plasmid?

I want to overexpress a mouse protein into mouse peritoneal macrophage, so which method will work best viral transduction or plamid transfection and if anybody has protocol for the same please let...

02 February 2014 6,285 6 View