I have a strain which my protein tagged with mNG and I wish to observe its expression using western blot. I wonder can I use GFP antibody to detect mNeonGreen signals? Do they have the same recognition site?
Hi,
I am not sure that ordinary anti-GFP antibodies will successfully target mNeonGreen, because regular GFPs or YFPs, such as VenusYFP, EGFP, mGFP5 ... are derived from Aequorea victoria GFP, which is a cnidaria, while mNeonGreenFP is derived from Branchiostoma lanceolata, which is a chordate.
Those two FPs must differ largely and I bet that commercial antibodies raised against jellyfish FPs will not recognize lancelet FPs.
Most of anti-GFP antibodies do not target succesfully FPs derived from corals or sea anemones.
Oliv
Hi,
Oliv is right. You need an antibody against mNeonGreen. anti-GFP antibodies do not recognize mNeonGreen.
Hi,
The above answers are correct. I tried with our anti-GFP antibody (JL-8 from Clontech), and it failed to detect my fusion protein tagged with mNGreen. However, the antibody did detect the eYFP which served as control. The company seems haven't developed
an antibody for mNGreen for Western blot (see http://www.allelebiotech.com/mneongreen-nab/)
Just got the information from my colleague. You can try the following antibody for mNGreen: http://www.chromotek.com/products/conventional-antibodies/mneongreen-antibody-32f6/
https://www.chromotek.com/products/detail/product-detail/mneongreen-antibody-32f6/
It worked really well in IF and WB.
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