18 Questions 31 Answers 0 Followers
Questions related from Yen Teng Tai
I extracted my brain organoid samples recently and ran them on SDS-PAGE. These brain organoid samples were coated with Matrigel, I tried to remove as many as I can by washing them in cold 1X PBS...
21 January 2022 8,541 4 View
I found that knockout of my gene of interest causes higher Wnt activity (as shown by TOP/FOP flash luciferase assay as well as RNA-seq) and hence, I am trying to figure out the mechanism that...
27 November 2021 4,454 3 View
Hi, I am currently trying to co-immunoprecipitate a protein but the interaction between that protein and its binding partner is not stable. Sometimes we able to detect its binding partner but...
30 November 2020 9,362 3 View
Has anyone tried to study Wnt signaling in HEK293T cells? I have been trying to measure the Wnt activity using TOP/FOP-FLASH assay but the readings that I got are rather similar in both TOP and...
29 August 2020 6,165 4 View
I have performed the ChIP and quantify the samples using Qubit Flurometer 2.0. Here are the results: Untagged control :2.72ng/mL Tagged sample: 3.17ng/mL I think the untagged strain should have...
10 April 2018 1,661 2 View
If overexpression of protein A can rescue the growth defect caused by mutation in protein B, can I consider protein A is working downstream of protein B? If yes, why is it so?
24 May 2017 6,133 23 View
I am preparing samples for BigDye sequencing. After PCR, I did ethanol precipitation to clean up the samples. In order to dry up the samples (after removing 70% ethanol), is it okay for me to dry...
25 October 2016 9,603 7 View
One of the proteins in S.pombe that I am working on is tagged with FLAG, so can I still call this strain as wild type as it contains a fragment of foreign DNA? If no, what is the right term for it?
02 June 2016 5,060 3 View
I have digested my PCR product with NEB Not1 and Nde1 for 1hr. I have cut the band and purified the product. However I afraid the digestion is incomplete, can I re-digest them again? Is there any...
24 May 2016 7,142 15 View
May I know 400U of lambda phosphatase can dephosphorylate how many microgram of protein? I am using lambda phosphatase from Cosmo Bio.
29 February 2016 5,124 4 View
May I know what is the maximum number of plasmids (with different origins of replication) can be taken up by a cell? What factors affect this? The maximum I have tried was two.
23 February 2016 8,683 4 View
I have ran 16S PCR on my metagenomic DNA samples (extracted from wastewater). However, I got multiple bands. Is this normal?
17 June 2015 1,473 18 View
The size of my gene is 1.7kb plus promoter is 800bp, so when I do PCR using plasmid primers, I should get 2300bp. However, I only can get 1500bp after I ran gel. I have screened through many...
22 July 2014 7,858 19 View
I have sent my soil sample for amplicon sequencing, the primers are targeted on bacteria V3-V5 region. However, the result that I obtained contains other things such as animalia, archae and...
03 December 2013 459 5 View
After I did blue-white screening, I picked the white colonies and subculture on LB/Amp plate. They can grow well but when I subculture again on new plate, they cannot grow. May I know the reason?...
24 October 2013 7,247 2 View
May I know if it is true that gene with any sequence also can be inserted into pGEM-T Easy? Since it has AT-overhang, so will this affect the type of gene inserted?
09 October 2013 1,490 15 View
I am extracting DNA from soil sample based on Zhou's conventional method but I am facing problems with the humic acids. The humic acids will decrease the purity of DNA and thus, inhibits the...
29 April 2013 8,965 3 View
the sample using gel electrophoresis. After that, I sonicate the sample but when I want to cut gel
25 April 2013 4,927 10 View
