How mirna chose its target among 100s of mRNA having seed sequence?

I am not clear what is rule behind mirna and mRNA targeting pair,because each mirna target officially 100s of target. In such cases how one will validate legitimate mirna mRNA target. Assays...

21 April 2019 281 4 View

Films prepared by combination of Hypromellose E15 and E5 polymer with 20% of plasticizer (PEG 400) w.r.t dry polymer using water results in brittle ?

This question is related to soluble film formulation.

04 September 2018 3,086 1 View

How to meassure primary miRNA trancript using QRT PCR?

I want to know how to design primer for primary miRNA QRT PCR

05 March 2018 4,842 6 View

How to draw Scatchard plot, using data of fluorescence intensity and concentrations of ligands and protein only?

How to draw Scatchard plot, using data of fluorescence intensity and concentrations of ligands and protein only. Please help..

22 January 2018 992 4 View

I wanted to see if all the morphs of nematodes are same species or not by molecular sequencing, how to proceed doing it?

20 March 2017 5,885 3 View

What is the function of acetic acid and methanol in staining-destaining of sds page?

function of acetic acid and methanol in staining-destaining of sds page

17 February 2017 3,392 4 View

Can miRNA mimics or inhibitor used in primary cells?

I am currently working with lymphocyte ,I want to know if any one has used the miRNA mimics or inhibitor with primery cells

02 November 2016 1,753 3 View

How to reduce size of a void in a metal from micron to nanometer range ??

I want to know if there is a way in which the void of the material inside the metal can be divided from micron size to nanometer size by splitting the voids into discrete voids of reduced size...

03 October 2016 6,811 3 View

How is the molecular phylogenetic analysis of foraminifera beneficial for taxonomic studies?

I would like to know the actual role of rDNA and protein sequences in evolutionary relationships of foraminifera.

23 August 2016 9,210 2 View

Has anyone implemented Self Organizing (Adaptive) Fuzzy controller?

I have read a lot theory about Self Organizing Fuzzy control from Internet. But I am still not completely able to understand its theory and implementation.I have attached one of the best document...

02 June 2016 2,438 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View