Can I leave blot in block buffer for 48 hours?

More Nahla Fadl's questions See All

What is the meaning of this error and how to solve it? - mike 21 - Coupled Model FM?

What is the meaning of this error and how to solve it? - mike 21 - Coupled Model FM

03 November 2023 6,170 1 View

Is it possible to have an increase of 5-HT immunoreactive neurons after a treatment in a region where we don't expect neurogenesis ?

is it due to the immunostaining?

31 October 2023 329 1 View

In a global economy knowledge may be a company's greeted competitive advantages why?

12 August 2023 9,056 2 View

What are the most important knowledge management systems?

25 July 2023 302 2 View

Is there a complementarity and overlap between the human and scientific sciences?

25 July 2023 9,709 1 View

What is the difference between ISI and Scopus Journals? ISI versus Scopus What are they?

Is it better to publish in Journals indexed ISI Or in Scopus ( Social Sciences)

06 July 2023 3,658 2 View

How to identify corals upto species level using wide-arrayed photographs (attached)?

Coral photographs lacking the corallite features hinders identification and I found it to be very much challenging. However, any tips regarding coral identification will be appreciated.

01 July 2023 7,373 0 View

What are the specifications of the suitable memristor to be used in analog circuits?

I want to choose between different memristor models and different physical realizations what is suitable to be used in analog applications

05 June 2023 6,766 0 View

Is there a type of patch normalization where the divider is usually integer of specified range? For example, in image/text recognition application?

We work on developing new technique for dividing by integers and its hardware implementation. we think in specify this technique to one of the processes needed in the machine learning field. Then...

18 May 2022 9,736 0 View

Can you determine the MIC based on the total polyphenols concentration (mg GAE/ml)?

After extracting my industrial fruit waste (dried 50g in 100ml distilled water) and measuring its TPC. I went straight into assessing its antibacterial activity. My extract is in liquid form (no...

09 April 2022 3,937 4 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

How can I improve the quality of lamb milk replacer for machine feeding?

Hello everyone, I am researching ways to enhance the quality of lamb milk replacer for machine feeding. I would appreciate any insights or recommendations on the following: Manual vs. Machine...

06 August 2024 6,227 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

I found the zeta potential of exosomes in DLS and it was -8mv. So is it correct or not ?

What should be the zeta potential of bovine milk exosomes

25 July 2024 7,376 5 View

Edward Michelini

Bacterial or even fungal growth could be a concern with long storage times in NFDM at 4C. Is there any preservative present (thimerosal, azide or kathon)?

If not the TBS-T, as Sebastian wrote, could be a better choice.

Nahla Fadl

Thank you both I could not change the block to TBS-T so I left it but I also left the antibody overnight and it worked.