Can I culture HEK293T cells and MSCs together (co-culture)? What would be the best method?
How can I extract RNA from HEK293T only to monitor gene expression from being co-cultured with MSCs (simply put, I want to monitor the effect of MSC through gene/protein expression)
Use cell culture inserts (transwell), you growth msc in a well plate and the hek in the inserts, as the inserts are removable you can trpsinize them or stain
If your cells mixed together, you might be able to trypsinize them and use a cell sorter to separate the two cell types so you could lyse them individually.
@Atia Hakim, another good option to avoid cell contamination is to harvest a culture supernatant (secretome) from MSC, centrifuge 500g 10mins, and add SN to Hek293T. Then just lyse Hek293T attached on the plate, this is the best option. I dont recommend trypsinizstion before RNA extraction. Good luck!
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