Theresa Smith

4 Questions 24 Answers 0 Followers

Questions related from Theresa Smith

Caco-2 transport assay - random TEER collapses?

I'm trying to develop a reliable protocol for a caco-2 transport assay, but I'm encountering a problem with the TEER not being stable over the course of the assay. I measure the TEER before I...

25 October 2023 7,046 1 View

What is the correct way to handle standard deviation when averaging values that are averages themselves?

Say I have multiple samples and I want to calculate the average amount of protein X present in each of them. However, to measure the quantity of protein X in each sample, I took three readings and...

15 June 2023 6,621 1 View

How to differentiate caco-2 cells on a regular culture surface without a transwell insert?

I have seen multiple sources suggesting that culturing Caco-2 cells in a flask or something like a 6-well plate for 14-21 days with media changes should cause them to form a differentiated...

19 May 2023 3,462 5 View

What is the best way to clear mammalian cell lysate for HPLC protein purification?

We frequently purify proteins from mammalian cells using HPLC, which requires a clarified lysate free from cell-debris. Our current method is to centrifuge the cell lysate at 33,000xg and then to...

16 March 2021 9,661 1 View