Theresa Smith

2 Questions 13 Answers 0 Followers

Questions related from Theresa Smith

How to differentiate caco-2 cells on a regular culture surface without a transwell insert?

I have seen multiple sources suggesting that culturing Caco-2 cells in a flask or something like a 6-well plate for 14-21 days with media changes should cause them to form a differentiated...

19 May 2023 3,376 5 View

What is the best way to clear mammalian cell lysate for HPLC protein purification?

We frequently purify proteins from mammalian cells using HPLC, which requires a clarified lysate free from cell-debris. Our current method is to centrifuge the cell lysate at 33,000xg and then to...

16 March 2021 9,533 1 View