Hello, I'm currently doing my master thesis and I'm new in the field of hydrogels. I've had success in making chitosan-starch hydrogels using glutaraldehyde as a crosslinking agent, but I want to change the latter for genipin, because I want to use in biomedical research and GA can be toxic. I got my genipin from Sigma-Aldrich and it says is insoluble in water, but I've read some papers that says its slightly soluble in it. I know it's soluble in organic solvents, but I don't know if it could affect the hydrogel outcome, so I wanted to dissolve it in water. Also, I read that to make a stock, it should be dissolved in DMSO (which is toxic to cells) and purged with gas, but I do not want to use another toxic reagent. Can I make a stock solution of genipin in water?
Brian Ignacio Rivas Tiznado , you could check out this review on genipin to see how others incorporated it into a number of biological materials: Article Genipin-chitosan hydrogels as biomedical and pharmaceutical aids
. It is well cited so there should be a related non-toxic method of preparation of interest to you.
- Badges
- Science topic