What is the half-life of TNF-alpha in vitro?

I'm designing a low-grade chronic treatment with this cytokine and I'm wondering how regularly to change the culture medium based on the half-life of the protein in an in vitro environment....

24 January 2021 5,988 2 View

I would like to know if there is any report of the average volume of the lung lining fluid in BALB/c mice ?

I would like to know the volume of lining fluid in mice

24 April 2019 8,716 3 View

How can I measure encapsulation efficiency?

Hi, everyone! I have a drug with Platinum that is fat soluble, but my nanoliposomes are in aqueous suspension, so what is un-entrapped should be precipitate. I would like to measure encapsulation...

25 February 2019 7,623 1 View

Creating a new coordinate system in PETREL?

22 July 2018 7,099 14 View

HEK293 Cell line detachment after being adhered for over 3 days?

08 December 2016 9,523 2 View

Problems with the films for western blot developing?

Hi everyone! I am having some troubles when developing my western blot membranes using the film system: the films become really dark (even it is difficult to distinguish the bands) when I put them...

15 November 2016 891 7 View

Problems with the films for western blot developing?

Hi everyone! I am having some troubles when developing my western blot membranes using the film system: the films become really dark (even it is difficult to distinguish the bands) when I put them...

10 November 2016 7,734 8 View

What adherent substratum is appropriate for T cells, without any calcium current triggering?

24 October 2016 5,021 2 View

Best LC column for Plant Metabolomics?

Hi all!Typically we have been using a C18 Reverse Phase column for our studies, unfortunately there is no retention of most polar metabolites and all elute at very early retention times. This is,...

06 June 2016 8,133 10 View

How can I do a metabolite extraction for soil microorganisms?

Dear colleagues! I am trying to figure out how to do an extraction for soil microorganisms for further metabolomic analyses. I am only interested in the microbiota part, so I would like to discard...

08 December 2014 463 5 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

A white mass inside the head of a cretaceous ant. What can it be?

I have a small piece of Burmese amber with a worker of the stem ant Gerontoformica sp. When focusing the head using incident light, it can be seen a white bilateral structure with several lateral...

26 August 2020 9,688 10 View

Are there any lists of insect and other invertebrate taxa described from Bogor (Buitenzorg), Indonesia?

In late 19th and early 20th centuries, many species of ants (Insecta: Hymenoptera: Formicidae) had been described from Bogor (Buitenzorg), Indonesia. I want to know any other insect and other...

26 August 2020 657 4 View

What recommendations for a GPS unit with at least 1 m accuracy?

I am mapping ant nest locations both locally (in populations) and in regions. I plan to do mapping and metapopulation analysis. Will use ERSI ArcGIS and perhaps Ramas programs. The ant nests are...

27 February 2020 4,027 2 View

Studies on the survival of ant-groups as a function of caste composition?

I was wondering if anyone ever studied the group-survival of ants as a function of group composition; For instance, suppose you have a group of N ants, composed of 3/4 of minor workers and 1/4 of...

13 February 2020 3,993 2 View

What's the best way to do PCR on bacteria living in bacteriocytes inside of insects?

I don't really have access to make a complicated process for this, but I was wondering if there was a way to conduct PCR on an entire group of cells if I already have primers specific to the...

04 February 2020 7,225 3 View

What's the best way to do PCR on bacteria living in bacteriocytes inside of insects?

I don't really have access to make a complicated process for this, but I was wondering if there was a way to conduct PCR on an entire group of cells if I already have primers specific to the...

01 February 2020 1,566 3 View

How to implement AntHocNet or AntNet in NS2.35 for wireless sensor network?

I want to implement the Ant colony algorithm for a wireless sensor network using ns2. But I'm not sure about the process of implementing that. Please provide information about how to implement ACO...

29 December 2019 6,765 3 View

Is Cataglyphis genus present in the Arabian Peninsula?

I found contradictory information about this genus and I would like to understand its correct geographical range.

13 December 2019 8,348 8 View

How to implement AntHocNet or AntNet in NS2.35 for wireless sensor network?

I want to implement the Ant colony algorithm for a wireless sensor network using ns2. But I'm not sure about the process of implementing that. Please provide information about how to implement ACO...

11 December 2019 871 3 View