Manojkumar Avaghade

6 Questions 30 Answers 0 Followers

Questions related from Manojkumar Avaghade

How to purify all the nucleic acid (DNA and RNA) associated protein from bacteria?

I want to study the change in profile of nucleic acid associated proteins from bacteria.

09 September 2016 1,538 2 View

Can anyone provide me a M.tb glutamine synthetase clonned in e.coli?

I was having M.tb glutamine synthetase clone but due to some unavoidable situations I lost those clone. If anyone has a M.tb glutamine synthetase clonned in e.coli, I would be very thankful if...

07 July 2016 2,288 2 View

What is the optimum irreproducible retention time of the peak in HPLC?

I am doing reverse phase HPLC using C18. Keeping the flow rate and composition of solvent constant, I should get my target peak at 14 min, but I am getting the peak sometime at 14 minutes sometime...

06 June 2014 886 4 View

Can anyone tell me what kind of interaction there is between SDS and proteins in SDS-PAGE?

Does SDS forms any kind of bond with the protein? Please help with my query.

05 May 2014 8,238 11 View

waht should be a control for RNA isolation?

I am working on RNA isolation. As a positive control for RNA what should i use so that i can be sure that the method for isolation that i am doing is giving me correct result.

11 November 2011 2,060 2 View

Help me with molecule docking

I want to dock a molecule onto a identified target site and see how strong the affinity is. And i also want to modify the molecule and see if i could increase the affinity

10 October 2011 3,436 15 View