Can Cylex Immuknow be used with canine cells?

17 December 2013 0 10K Report

I can't find a protocol to measure dog blood cells response to PHA. I wish I could use Cylex Immuknow but the anti CD4 step may be unreliable with canine cells.

Badges
Science topic

Similar topics
Eutheria
Rodentia
Muridae
Murinae
Mice

More Delphine Grezel's questions See All

NBRIP assay: disappearance of the initial halo?

Dear colleagues, We are starting NBRIP assays in the lab to evaluate the capacity of rhizobacterial strains of our interest to solubilize calcium phosphate. To our surprise, the yellow/destained...

21 December 2021 1,309 0 View

What are the typical markers for phosphate and nitrogen starvation in Arabidopsis roots and shoots?

I am new in the field of plant nutrition and I am searching for the most relevant markers to use to follow N and P starvation in Arabidopsis (both in shoots and roots). What are the most sensitive...

08 November 2021 8,135 3 View

Tissue extraction from cryomold ?

Hi everyone, I received a piece of operative tissue in a frozen "cryomold". Im a not familiar with this thing. I wanted to do RNA extraction from this tissue. I don't know how to recover the...

08 August 2021 2,584 0 View

How to revive a Hyphomicrobium strain?

Dear peers, I need some advice to revive strains of Hyphomicrobium that have been stored at -80°C as glycerol stocks (as part of a reference stock). I have tried to culture them again on plates...

11 February 2021 3,753 2 View

Exosome quantification by Elisa based method ?

Hi, I'm working on exosomes isolated from blood samples and cell culture supernatant. I'm wondering if I could quantify exosomes based on Elisa Method coated with exo Ab (CD63 / CD9/C81...) My...

11 November 2020 835 3 View

About exosomes western blotting ?

Hi, I'm working on exosomes isolated from cell supernatant of cultured cells. Briefly, I accumulate 100 - 200 ml of supernatant and process it until a concentration in a final volume of 50µL....

20 July 2020 1,905 7 View

Will you want contrubutions from outside?

To know if you plan to add videos from other laboratories, so that the channel has several types of techniques - like the operation of SEM, XRD, GIXRD, AFM ... Good idea anyway!

09 April 2020 3,946 0 View

Has anyone used qSonica to shear DNA prior preparation for WGS library?

Hi everyone, I would like to fragment DNA of dolphins using qSonica to obtain fragment size between 300-350bp in order to prepare a library for Whole-Genome-Sequencing. I have already run...

26 February 2020 5,197 0 View

How to do RNA Pull Down ?

Hello, I'm trying to capture viral RNA from my total RNA fraction so i've extracted RNA with trizol and digest with DNase. Then I've design 7 RNA-probes of 40 nucleotide each to span all the...

24 February 2020 7,171 2 View

Ventilation de la terminologie de domaines de spécialité par catégorie grammaticale?

Je suis à la recherche de statistiques sur la ventilation par catégorie grammaticale (ou partie du discours) de la terminologie de différents domaines, dans le but de faire ressortir les...

28 May 2019 5,076 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View