How to revive a Hyphomicrobium strain?

11 February 2021 3,524 2 View

What part of heart ?

Hello You can see a transversal slice of a mouse heart in microscopie with blue coloration. My question : what part of the heart is it ? how i can recognize difference between histology of...

01 December 2020 1,000 3 View

Exosome quantification by Elisa based method ?

Hi, I'm working on exosomes isolated from blood samples and cell culture supernatant. I'm wondering if I could quantify exosomes based on Elisa Method coated with exo Ab (CD63 / CD9/C81...) My...

11 November 2020 664 3 View

About exosomes western blotting ?

20 July 2020 1,688 7 View

TiO2 nanoparticles (300nm) are clumping to form huge aggregates?

When added to Distilled water (not degassed or deoxygenated) my 300 nm TiO2 particles finely disperse to form a milk white mixture. However, when almost anything is added to this mixture (copper...

23 January 2018 2,333 5 View

Which antibodies anti-MSH6 and anti-PMS2 for Western blot analysis on human cell lines ?

Hi everyone, I am new in the field of Western Blot. I would like to perform Western Blot analysis after protein extraction on human cell lines. My two proteins of interest are MSH6 and PMS2. Which...

13 November 2017 5,079 3 View

How can we characterize healthy elderly?

When studying some metabolic parameters in elderly people, it seems difficult to distinguish which ones are caused by aging per se or by age-related diseases. In particular, circulating IL-6 is...

19 June 2014 5,373 5 View

How to detach adherent insect cells?

I have started some insect cells cultures and decided to go with adherent cultures. The protocol that came with the cells (Sf9) presented both the adherent and suspension cultures methods so I did...

25 December 2013 314 10 View

Does anyone know literature about an immune tolerance process to allogeneic father antigens in multiparous female rodents?

Along with the allogeneic foetus tolerance at the maternal interface is there some remanence of this mechanism that renders the mother less reactive when receiving a graft of father cells?

28 November 2013 9,288 3 View

Issue with estradiol inucble system in Arabidopsis

We are working with XVE system from Chua's lab and have encountered a serious problem of induction with estradiol: inducibility of trangene expression in whole leaves from adult plants is...

28 June 2012 9,898 7 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View