Exosome quantification by Elisa based method ?

Hi, I'm working on exosomes isolated from blood samples and cell culture supernatant. I'm wondering if I could quantify exosomes based on Elisa Method coated with exo Ab (CD63 / CD9/C81...) My...

11 November 2020 664 3 View

About exosomes western blotting ?

20 July 2020 1,688 7 View

Which antibodies anti-MSH6 and anti-PMS2 for Western blot analysis on human cell lines ?

Hi everyone, I am new in the field of Western Blot. I would like to perform Western Blot analysis after protein extraction on human cell lines. My two proteins of interest are MSH6 and PMS2. Which...

13 November 2017 5,079 3 View

Potassium acetate role in the aluminum chloride reaction?

I read an article where the Aluminum Chloride Method is used for the determination of total flavonoid content after the reaction between the sample and AlCl3 took place they add potassium acetate....

20 September 2017 3,009 2 View

How can we characterize healthy elderly?

When studying some metabolic parameters in elderly people, it seems difficult to distinguish which ones are caused by aging per se or by age-related diseases. In particular, circulating IL-6 is...

19 June 2014 5,373 5 View

How to detach adherent insect cells?

I have started some insect cells cultures and decided to go with adherent cultures. The protocol that came with the cells (Sf9) presented both the adherent and suspension cultures methods so I did...

25 December 2013 314 10 View

Does anyone know literature about an immune tolerance process to allogeneic father antigens in multiparous female rodents?

Along with the allogeneic foetus tolerance at the maternal interface is there some remanence of this mechanism that renders the mother less reactive when receiving a graft of father cells?

28 November 2013 9,288 3 View

Issue with estradiol inucble system in Arabidopsis

We are working with XVE system from Chua's lab and have encountered a serious problem of induction with estradiol: inducibility of trangene expression in whole leaves from adult plants is...

28 June 2012 9,898 7 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View