Hi everyone,

I would like to fragment DNA of dolphins using qSonica to obtain fragment size between 300-350bp in order to prepare a library for Whole-Genome-Sequencing. I have already run several trials changing the sonicating time from 30s up to 5min30s with current results suggesting that shearing for 90s will be best. However, instead of getting a pick around 300-350bp, I got a smear from 700bp approx.

Unfortunately, I don't have much DNA to spare (limited with amount of extracted DNA and concentration), so any advice or even example of anyone who would have done it will be wonderful to better target any other tests I should be doing.

Thanks in advance for responding and your help.