How to revive a Hyphomicrobium strain?

11 February 2021 3,524 2 View

Exosome quantification by Elisa based method ?

Hi, I'm working on exosomes isolated from blood samples and cell culture supernatant. I'm wondering if I could quantify exosomes based on Elisa Method coated with exo Ab (CD63 / CD9/C81...) My...

11 November 2020 664 3 View

About exosomes western blotting ?

20 July 2020 1,688 7 View

Which antibodies anti-MSH6 and anti-PMS2 for Western blot analysis on human cell lines ?

Hi everyone, I am new in the field of Western Blot. I would like to perform Western Blot analysis after protein extraction on human cell lines. My two proteins of interest are MSH6 and PMS2. Which...

13 November 2017 5,079 3 View

Potassium acetate role in the aluminum chloride reaction?

I read an article where the Aluminum Chloride Method is used for the determination of total flavonoid content after the reaction between the sample and AlCl3 took place they add potassium acetate....

20 September 2017 3,009 2 View

How can we characterize healthy elderly?

When studying some metabolic parameters in elderly people, it seems difficult to distinguish which ones are caused by aging per se or by age-related diseases. In particular, circulating IL-6 is...

19 June 2014 5,373 5 View

How to detach adherent insect cells?

I have started some insect cells cultures and decided to go with adherent cultures. The protocol that came with the cells (Sf9) presented both the adherent and suspension cultures methods so I did...

25 December 2013 314 10 View

Does anyone know literature about an immune tolerance process to allogeneic father antigens in multiparous female rodents?

Along with the allogeneic foetus tolerance at the maternal interface is there some remanence of this mechanism that renders the mother less reactive when receiving a graft of father cells?

28 November 2013 9,288 3 View

Issue with estradiol inucble system in Arabidopsis

We are working with XVE system from Chua's lab and have encountered a serious problem of induction with estradiol: inducibility of trangene expression in whole leaves from adult plants is...

28 June 2012 9,898 7 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View