BT549- Tail vein injection?

Hi everyone, does anyone have experience with performing tail vein injection with the breast cancer cell line BT549? I am looking for some advice on what the metastasis burden looks like, what...

14 October 2020 215 2 View

How to Identify and Estimate the Lactic acid from the Microbial fermented broth other than HPLC using Simple methods?

How to Identify and Estimate the Lactic acid from the Microbial fermented broth other than HPLC using Simple methods? if possible such as 1. Calorimetric or Spectroscopic methods and much more

26 August 2020 349 10 View

Suggest some journals for research proposal?

I have selected the topic Drought assessment using remote sensing and GIS. Please kindly recommend some journals for better understanding the topic.

15 June 2020 3,104 3 View

Recent topics in GIS and Remote sensing ?

I need a topic for my Major dissertation work in the field of GIS and Remote sensing . i want to show my programming language skills also. i need current innovative topic .Can anyone suggest me...

30 March 2020 2,761 26 View

Please point me to sites where I can find health related research papers ?

The challenge is to create a model that uses data from the first 24 hours of intensive care to predict patient survival . My dataset has more than 130,000 hospital Intensive Care Unit (ICU) visits...

23 February 2020 3,583 2 View

How do devices live in their own reality based on the information that has been derived?

This question is for my thesis which exploring the internet infrastructure, internet of things and aspects of object-oriented ontology in building device-device relationships.

22 September 2019 2,367 2 View

Conversion of ppm gor NH3 gas to micromole/g ?

I use ammonia of 0.3g in a TPD experiment. I get 21.02 ppm of NH3 which I intend to convert it to micromole/g of NH3. I have got some feedback from professors but it seems to be a little...

02 July 2019 4,828 5 View

What are the changes in the plasma during co-sputtering?

I am currently working on optimization of TiNi thin film deposition using magnetron sputtering. I was able to obtain a free standing film using a single target with the following parameters. Ar...

23 October 2018 782 7 View

Inhibitors of EGFR internalization?

In our lab, to block internalization and endocytosis of EGFR, an inhibitor solution of 2 mM of sodium fluoride (NaF), 10 mM of sodium azide (NaN3) and 5 mM of 2-deoxy-D-glucose is used. Someone...

08 October 2018 4,646 3 View

How to get free standing NiTi Thin films?

Hi all, I am trying to get a free standing NiTi by depositing a layer of copper between a Si substrate and the film but when I treat the substrate with HNo3 the film just breaks, I think the film...

20 August 2018 7,363 7 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View