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In our lab, to block internalization and endocytosis of EGFR, an inhibitor solution of 2 mM of sodium fluoride (NaF), 10 mM of sodium azide (NaN3) and 5 mM of 2-deoxy-D-glucose is used. Someone...
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ATCC website says CHO-K1 cells require proline in their growth medium. https://www.atcc.org/products/all/CCL-61.aspx#characteristics We have been culturing CHO-K1 cells in our lab for many years...
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