In our lab, to block internalization and endocytosis of EGFR, an inhibitor solution of 2 mM of sodium fluoride (NaF), 10 mM of sodium azide (NaN3) and 5 mM of 2-deoxy-D-glucose is used.
Someone started using it many years ago and everyone else just followed. Nobody seems to know why this particular composition is used. Why are these particular salts used and how do they actually work? Are there better alternatives?
This mixture appears to be non specific in blocking internamlization and endocytic degradation of EGFR. It has been rather added to buffers or culture media to deplete ATP and inhibit apoptosis in some studies.
Indeed, sodium fluoride (NaF) is inhibitor of glycolysis, sodium azide (NaN3) is known as inhibitor of terminal oxidation, and 2-deoxy-D-glucose seems to be inhibitor of glucose uptake.
This mixture appears to be non specific in blocking internamlization and endocytic degradation of EGFR. It has been rather added to buffers or culture media to deplete ATP and inhibit apoptosis in some studies.
Indeed, sodium fluoride (NaF) is inhibitor of glycolysis, sodium azide (NaN3) is known as inhibitor of terminal oxidation, and 2-deoxy-D-glucose seems to be inhibitor of glucose uptake.
I agree with Vehary.
2-DG actually blocks glucose metabolism by saturating hexokinase in competition with glucose. It depletes ATP by depleting glucose availability.
At a certain concentration it is pro-apoptotic.
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