I work with zebrafish embryos that express APEX2, and when I soak them with Biotin-tyramide in DMSO (10% for 2 hours, etc.) they die.
is there a less toxic way to quench the embryos with biotin?
I found that almost every paper that shows in situ hybridization, uses DIG-UTPs for labeling with fluorescence anti-DIG antibodies. Is there any advantage over Biotin-UTPs and fluorescence avidin...
21 March 2023 2,412 0 View
How do I know if my in situ hybridization probes binds mRNA and not genomic locus?
18 March 2023 2,057 7 View
Hi I'm looking for the subcellular location of some maternal transcripts in zebrafish embryos, before the genome activation (MZT). I use 'Molecular Instruments' HCR-FISH kit (probes and...
17 March 2023 2,418 0 View
I have a plasmid that I want to delete a segment from it. I designed primers for linearization of the vector without the unwanted sequence, and with overlapped ends. I heard that Gibsson assembly...
17 January 2023 7,942 4 View
Octakis(trimethylsiloxy)silsesquioxane is reported to be sparingly soluble (0.61 g/L 25°C Calc). I tried using organic solvent such as toluene, hexane, DCM and chloroform, however the powder...
13 January 2021 1,323 3 View
Hello all, I have TEMPO radical and I want to manipulate it in a way the it will no longer be a stable radical, do you know if that can be done? Alternatively, do you know of a material to TEMPO...
14 June 2020 4,455 0 View
I have stuck frit inside an HPLC column I want to remove, anyone knows how to remove it?
16 February 2019 5,876 6 View
Hello all, I'm using an home-built HPLC column and the pressure goes to the maximum even with very low flow (0.01 ml/min). Do you have any suggestions as to how to reduce pressure? Perhaps I...
03 September 2018 7,763 2 View
the silica nanoprticles may be dissolved to sodium silicate
07 July 2018 1,468 3 View
Is increasing large scale fading coefficients across the cells causes pilot contamination in massive MIMO? When orthogonal pilots are used across cell?
22 January 2018 7,523 4 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
I intend to get an economical and accurate LAB TEST to measure arsenic concentration in blood sample.
23 July 2024 3,654 2 View
I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...
19 July 2024 8,522 0 View
I am conducting free radical polymerization using Kraft lignin and MMA, with DMSO serving as the solvent and there is also an initiator. Following the reaction, I obtain a solution, but I am...
15 July 2024 9,827 1 View
We have been using MFC to control the flow rate of pure ammonia gas. The MFC is placed in an oven at 50 deg. C to avoid blockage due to NH3. However, after a few days of usage, the MFC is unable...
11 July 2024 5,796 1 View
I'm using Dorsomorphin (C24H25N5O) as one component in dual SMAD inhibition of pluripotent stem cells to differentiate into neurons alongwith SB431542. Now as to solubilizing 10mg SB in DMSO for...
11 July 2024 4,832 0 View
I have a very polar alcohol (soluble in MeOH, H2O, partially in heated DMSO). And a long chain fatty acid that I would like to couple. I can't find a common solvent for them. I can form the...
24 June 2024 9,319 0 View
I saw the iPSC about a week after holding the stock (12 well and 3 stocks) and there were very few cells attached, and some cells were differentiated. I wonder what the problem is. The plate is...
12 June 2024 6,087 0 View