Hello all,
I'm using an home-built HPLC column and the pressure goes to the maximum even with very low flow (0.01 ml/min).
Do you have any suggestions as to how to reduce pressure?
Perhaps I should pack the column with more / less stationary phase?
The stationary phase is silica-gel embedded with magnetite and the mobile phase is water.
Thank you!
Shorten the column. Replace the packing with a material of larger particle size. Be aware that both of these routes will affect the separation of materials in a negative fashion.
Besides Prof. Rawle suggestions, I recommend the followings:
1- Check the frits to be sure they aren't clogged.
2- Increase the column temperature.
3- Add a less polar solvent (e.g. acetonitrile) to the mobile phase (if possible!)
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