I have stuck frit inside an HPLC column I want to remove, anyone knows how to remove it?
Connect up your HPLC pump line to the column fitting and, while directing the end fitting with the stuck frit into a beaker. Use the flow to "push" the frit out. *We use this trick a lot when re-packing columns (pump IPA).
In addition to Bill's suggestion be sure to use a 'low' flow rate! Otherwise the column packing will come out too.
No column packing will be present when trying to remove the frit. This process is performed on the end-fitting, not the column.
I tried and it didn't work
dont let it to dry in the column, remove with pressure while it is wet.
Sonicate the end fitting to loosen it, then apply flow.
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